Identification of homocystinuric newborn is hindered by the pitfalls of neonatal sceening programs. We propose a fluorimentric HPLC method with a rapid pre-analytica step for homocysteine determination from neonatal dried blood spot cards. Homocysteine in blood spots sampled among 2000 healthy newborns on living day 4, averaged 2.92 ± 2.07 mM (range 0.4-7.5). In eight homocystinuric control children, mean values were 61.71 ± 52.84 mM (range 18.9-145.7). The method showed a good linearity (r = 0.999), precision (RDS<7%) and recovery (95%). The correlation between blood spots and rapid pre-analytical step combined with method linearity and precision.
Newborn screening of homocystinuria: quantitative analysis of total homocyst(e)ine on dried blood spot by liquid chromatohraphy with fluorimetric detection
2003
Abstract
Identification of homocystinuric newborn is hindered by the pitfalls of neonatal sceening programs. We propose a fluorimentric HPLC method with a rapid pre-analytica step for homocysteine determination from neonatal dried blood spot cards. Homocysteine in blood spots sampled among 2000 healthy newborns on living day 4, averaged 2.92 ± 2.07 mM (range 0.4-7.5). In eight homocystinuric control children, mean values were 61.71 ± 52.84 mM (range 18.9-145.7). The method showed a good linearity (r = 0.999), precision (RDS<7%) and recovery (95%). The correlation between blood spots and rapid pre-analytical step combined with method linearity and precision.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
