Recirculation factor is a key parameter in continuous-flow biomass selection for PHA production

The effectiveness of polyhydroxyalkanoates (PHA) production with mixed microbial cultures (MMC) largely depends on the selection of PHA-storing microorganisms, conventionally performed in sequencing batch reactors (SBR). These, although easily allow the establishment of the required feast and famine (FF) regime, can representa factor of cost increase when the process is scaled up. Here, a novel continuous-flow process for MMC selection under FF conditions has been developed by using two sequentially operated reactors. The feast reactor, having a tubular configuration, was continuously fed with a synthetic mixture of acetic and propionic acids (at an organicloading rate of 2.12 gCOD/L d) and the effluent of this reactor was in part sent to the CSTR famine reactor. The recirculation factor (RC), that is the ratio between the recirculation flow rate and the feeding flow rate to the feast reactor, was the main parameter investigated. Four different runs were performed with the RC varying from 1 to8 and the increase in its value caused a decrease of the biomass residence time in each reactor. The intracellular PHA content in the feast reactor almost linearly increased up to RC 4 (with a value of 34 ± 2 %, wt/wt) and dropped at the RC 8 condition that, however, showed the maximum PHA content (58 ± 5 %, wt/wt) during the accumulation tests. Indeed, the relative abundance of sequences affiliated with putative PHA-storing bacteria increased up to 90.5 % at RC 8 and were dominated by members of the Alphaproteobacteria class mostly represented by the genus Meganema (74 %)