Microorganisms living in extreme environments represent an important source for novel biocatalysts suitable for industrial processes. During the last few years, the evolution of high-throughput DNA sequencing has enabled the development and improvement of sequence-based metagenomic approaches that overcome the frequently observed limitations in the cultivation of extremophiles under laboratory conditions. We have exploited this approach in analyzing different high-temperature ecosystems like hot springs and we found novel enzymes with useful synthetic applications, such as epoxide hydrolases[1-3] and amine transferases[4]. More recently, the complementary use of activity-guided methods as an alternative discovery approach, such as enrichment cultures, led us to identification of a novel thermostable ?-amino acid transaminase from a Meiothermus strain isolated in an Icelandic hot spring showing a broad substrate scope[5]. Additionally, we have recently discovered a library of novel hydroxysteroid dehydrogenases, enzymes so far described only from gut and soil bacteria, by mining extremophilic (meta)genomes. The results obtained in the study of the substrate promiscuity and synthetic application of these novel biocatalysts with non-steroidal substrates will be presented [6,7]. References [1] E. E. Ferrandi et al., FEBS Journal, 282, 2879-2894 (2015), DOI: 10.1111/febs.13328. [2] E. E. Ferrandi, et al., ChemCatChem, 7, 3171-3178 (2015), DOI: 10.1002/cctc.201500608. [3] E. E. Ferrandi, et al., Front. Bioeng. Biotechnol., 6, 144 (2018), DOI: 10.3389/fbioe.2018.00144. [4] E. E. Ferrandi, et al., Appl. Microbiol. Biotechnol., 101, 4963-4979 (2017), DOI: 10.1007/s00253-017-8228-2. [5] E. E. Ferrandi, et al., Biotechnol. J., 15, 2000125 (2020), DOI: 10.1002/biot.202000125. [6] S. Bertuletti, et al., Adv. Synth. Catal., 362, 2474-2485 (2020), DOI: 10.1002/adsc.202000120. [7] S. Bertuletti, et al., Eur. J. Org. Chem., (2021) in press, DOI: 10.1002/ejoc.202100174.
Mining extremophiles (meta)genomes for novel and thermostable biocatalysts
Daniela Monti
2021
Abstract
Microorganisms living in extreme environments represent an important source for novel biocatalysts suitable for industrial processes. During the last few years, the evolution of high-throughput DNA sequencing has enabled the development and improvement of sequence-based metagenomic approaches that overcome the frequently observed limitations in the cultivation of extremophiles under laboratory conditions. We have exploited this approach in analyzing different high-temperature ecosystems like hot springs and we found novel enzymes with useful synthetic applications, such as epoxide hydrolases[1-3] and amine transferases[4]. More recently, the complementary use of activity-guided methods as an alternative discovery approach, such as enrichment cultures, led us to identification of a novel thermostable ?-amino acid transaminase from a Meiothermus strain isolated in an Icelandic hot spring showing a broad substrate scope[5]. Additionally, we have recently discovered a library of novel hydroxysteroid dehydrogenases, enzymes so far described only from gut and soil bacteria, by mining extremophilic (meta)genomes. The results obtained in the study of the substrate promiscuity and synthetic application of these novel biocatalysts with non-steroidal substrates will be presented [6,7]. References [1] E. E. Ferrandi et al., FEBS Journal, 282, 2879-2894 (2015), DOI: 10.1111/febs.13328. [2] E. E. Ferrandi, et al., ChemCatChem, 7, 3171-3178 (2015), DOI: 10.1002/cctc.201500608. [3] E. E. Ferrandi, et al., Front. Bioeng. Biotechnol., 6, 144 (2018), DOI: 10.3389/fbioe.2018.00144. [4] E. E. Ferrandi, et al., Appl. Microbiol. Biotechnol., 101, 4963-4979 (2017), DOI: 10.1007/s00253-017-8228-2. [5] E. E. Ferrandi, et al., Biotechnol. J., 15, 2000125 (2020), DOI: 10.1002/biot.202000125. [6] S. Bertuletti, et al., Adv. Synth. Catal., 362, 2474-2485 (2020), DOI: 10.1002/adsc.202000120. [7] S. Bertuletti, et al., Eur. J. Org. Chem., (2021) in press, DOI: 10.1002/ejoc.202100174.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
