Background. Acute T-cell mediated rejection (aTCMR) is still an issue in kidney transplantation for it is associated with chronic rejection, graft loss, and overall worse outcomes. For these reasons, a standard non-invasive molecular tool to detect is desirable to offer a simpler monitoring of kidney transplant recipients (KTRs). The purpose of our study was to examine in peripheral blood, before and after transplantation, the expression patterns of Treg-related genes, FOXP3 and the two CTLA-4 isoforms (full-length and soluble), to predict aTCMR onset, de novo donor-specific antibodies (DSA) development and renal dysfunction. Methods. We profiled by using a qRT-PCR method the circulating mRNA levels of these biomarkers in peripheral blood of 89 KTRs within the first post-transplant year (baseline, at day 15, 60, 365 and when possible at aTCMR) and compared the results with 24 healthy controls. Results. The three mRNA levels drastically reduced 15 days after transplantation and gradually recovered at one year in comparison with baseline, with very low levels by the time of aTCMR for FOXP3 (RQ=0.445, IQR=0.086-1.264, p=0.040). Healthy controls exhibited higher FOXP3 levels than patients at baseline (median RQ: 2.132, IQR=1.664-2.895 vs. aTCMR-free pts RQ:1.630, IQR=1.072-2.367, p=0.005). Noteworthy, solCTLA-4 displayed a dual profile: on the one hand, at multivariate analysis solCTLA-4 transcripts at 15 days were associated with an increased risk of aTCMR over time (HR=3.905, 95%CI: 0.958-15.916, p=0.050), and graft dysfunction at one year (AOR=3.683, 95%CI=1.165-12.079, p=0.027); on the other hand, pre-transplant levels showed a protective association with de novo (DSAs) development (HR=0.189, 95%CI=0.078-0.459, p<0.001). Conclusions. mRNA levels of Treg-associated genes, mainly for solCTLA-4 in peripheral blood might help shape immunosuppression, tailor monitoring and achieve better long-term outcomes of kidney transplantation in the wake of "precision medicine".
Monitoring of molecular profiling of regulatory T cell biomarkers by using non-invasive strategies to predict outcome in renal transplantation
Canossi A;
2021
Abstract
Background. Acute T-cell mediated rejection (aTCMR) is still an issue in kidney transplantation for it is associated with chronic rejection, graft loss, and overall worse outcomes. For these reasons, a standard non-invasive molecular tool to detect is desirable to offer a simpler monitoring of kidney transplant recipients (KTRs). The purpose of our study was to examine in peripheral blood, before and after transplantation, the expression patterns of Treg-related genes, FOXP3 and the two CTLA-4 isoforms (full-length and soluble), to predict aTCMR onset, de novo donor-specific antibodies (DSA) development and renal dysfunction. Methods. We profiled by using a qRT-PCR method the circulating mRNA levels of these biomarkers in peripheral blood of 89 KTRs within the first post-transplant year (baseline, at day 15, 60, 365 and when possible at aTCMR) and compared the results with 24 healthy controls. Results. The three mRNA levels drastically reduced 15 days after transplantation and gradually recovered at one year in comparison with baseline, with very low levels by the time of aTCMR for FOXP3 (RQ=0.445, IQR=0.086-1.264, p=0.040). Healthy controls exhibited higher FOXP3 levels than patients at baseline (median RQ: 2.132, IQR=1.664-2.895 vs. aTCMR-free pts RQ:1.630, IQR=1.072-2.367, p=0.005). Noteworthy, solCTLA-4 displayed a dual profile: on the one hand, at multivariate analysis solCTLA-4 transcripts at 15 days were associated with an increased risk of aTCMR over time (HR=3.905, 95%CI: 0.958-15.916, p=0.050), and graft dysfunction at one year (AOR=3.683, 95%CI=1.165-12.079, p=0.027); on the other hand, pre-transplant levels showed a protective association with de novo (DSAs) development (HR=0.189, 95%CI=0.078-0.459, p<0.001). Conclusions. mRNA levels of Treg-associated genes, mainly for solCTLA-4 in peripheral blood might help shape immunosuppression, tailor monitoring and achieve better long-term outcomes of kidney transplantation in the wake of "precision medicine".I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
