Reversed phase capillary electrochromatography in 5 mm C18 fully packed capillary was employed to optimize the separation of negatively charged non steroidal antiinflammatory drugs. The effect of physico-chemical parameters and different analysis modes on APAs separation were studied and evaluated. The mobile phase composition, buffer type, concentration and pH differently influenced the peak efficiency and resolution, selectively modulating the analytes interaction with the stationary phase. The use of zwitterionic MES or acetate mobile phases strongly modulated the analytes migration order and peak efficiency. The optimum experimental conditions were found in MES buffer pH 5.0 containing the 75% of acetonitrile/methanol mixture (1:1). All the analytes were baseline separated in mixture in less than 13 min with peak efficiencies in the range of 78,467-84,217 N/m. Under these conditions the analytes were negatively charged and their effective electrophoretic mobility played a role in the separation. The analysis of different pharmaceutical preparations containing antiinflammatory drugs, e.g. drops and tablets, was also presented after a very simple sample pretreatment.
Separation of negatively charged non steroidal antiinflammatory drugs by reversed phase capillary electrochromatography.
Desiderio C
2003
Abstract
Reversed phase capillary electrochromatography in 5 mm C18 fully packed capillary was employed to optimize the separation of negatively charged non steroidal antiinflammatory drugs. The effect of physico-chemical parameters and different analysis modes on APAs separation were studied and evaluated. The mobile phase composition, buffer type, concentration and pH differently influenced the peak efficiency and resolution, selectively modulating the analytes interaction with the stationary phase. The use of zwitterionic MES or acetate mobile phases strongly modulated the analytes migration order and peak efficiency. The optimum experimental conditions were found in MES buffer pH 5.0 containing the 75% of acetonitrile/methanol mixture (1:1). All the analytes were baseline separated in mixture in less than 13 min with peak efficiencies in the range of 78,467-84,217 N/m. Under these conditions the analytes were negatively charged and their effective electrophoretic mobility played a role in the separation. The analysis of different pharmaceutical preparations containing antiinflammatory drugs, e.g. drops and tablets, was also presented after a very simple sample pretreatment.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
