The simple and straightforward recognition of Triticum species is not an easy task due totheir complex genetic origins. To provide a recommendation, we have compared the performance ofdifferent PCR-based methods relying on the discrimination ability of the Q- and g-gliadin (GAG56D)genes, as well as TBP (Tubulin-Based Polymorphism), a method based on the multiple amplificationof genes of the -tubulin family. Among these approaches, the PCR-RFLP (Restriction FragmentLength Polymorphism) assay based on a single-nucleotide polymorphism (SNP) present in the Qgene is the only one capable of fully discerning hexaploid spelt and common wheat species, whileboth g-gliadin and TBP fail with similar error frequencies. The Q-locus assay results in the attainmentof either a single fragment or a doublet, depending on the presence of a suitable restriction site, whichis affected by the mutation. This dual pattern of resolution limits both the diagnostic effectiveness,when additional Triticum species are assayed and compared to each other, and its usefulness, whencommercially available flours are analyzed. These limitations are overtaken by flanking the Q-locusassay with the TBP analysis. In this way, almost all of the Triticum species can be accurately identified.
il lavoro descrive come l'utilizzo combinato del locus Q e del marcatore molecolare TBP conenta l'identificazione delle diverse specie commerciali di Triticum, incluso il farro spelta
A Combinatorial Q-Locus and Tubulin-Based Polymorphism (TBP) Approach Helps in Discriminating Triticum Species
Guadalupi C;Braglia L;Gavazzi F;Morello L;Breviario D
2022
Abstract
The simple and straightforward recognition of Triticum species is not an easy task due totheir complex genetic origins. To provide a recommendation, we have compared the performance ofdifferent PCR-based methods relying on the discrimination ability of the Q- and g-gliadin (GAG56D)genes, as well as TBP (Tubulin-Based Polymorphism), a method based on the multiple amplificationof genes of the -tubulin family. Among these approaches, the PCR-RFLP (Restriction FragmentLength Polymorphism) assay based on a single-nucleotide polymorphism (SNP) present in the Qgene is the only one capable of fully discerning hexaploid spelt and common wheat species, whileboth g-gliadin and TBP fail with similar error frequencies. The Q-locus assay results in the attainmentof either a single fragment or a doublet, depending on the presence of a suitable restriction site, whichis affected by the mutation. This dual pattern of resolution limits both the diagnostic effectiveness,when additional Triticum species are assayed and compared to each other, and its usefulness, whencommercially available flours are analyzed. These limitations are overtaken by flanking the Q-locusassay with the TBP analysis. In this way, almost all of the Triticum species can be accurately identified.File | Dimensione | Formato | |
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