Photochromic FRET (pcFRET) combines the super-resolution capability of FRET technique with the photomodulation of the optical characteristics of the acceptor chromophore, in view of obtaining a fast and reliable assessment of molecular binding in solution spectroscopy and fluorescence microscopy. This paper summarizes the main photophysical features of pcFRET and describes a model to analyze obtainable data. © 2023 Società Italiana di Fisica. All rights reserved.
Theory of photochromic Forster energy transfer
Storti, Barbara;Bizzarri, Ranieri
2023
Abstract
Photochromic FRET (pcFRET) combines the super-resolution capability of FRET technique with the photomodulation of the optical characteristics of the acceptor chromophore, in view of obtaining a fast and reliable assessment of molecular binding in solution spectroscopy and fluorescence microscopy. This paper summarizes the main photophysical features of pcFRET and describes a model to analyze obtainable data. © 2023 Società Italiana di Fisica. All rights reserved.File in questo prodotto:
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