The need for measuring polyphenols during the vinification process is an old story. Today the most up-to-date non-destructive instrument for wine and must used in wineries is the FOSS, Winescan, based on FT-IR technology. But wineries need something which measures polyphenols during the extraction in order to automatize the process. We have followed two lines of research: one is to develop a low cost micro-NIR instrument and the other the development of a biosensor based on protein binding to polyphenol. In the first case, a homemade, low cost, Vis-NIR micro sensor on Arduino platform was used. Spectra have been acquired on different grape must cultivars, wines and chemometrics, to elaborate the correlation of spectra with destructive analyses, revealed an acceptable level of confidence but still not completely reliable. The other research is based on the application of SAW (surface acoustic waves) with selected protein to bind polyphenols. The SAW resonator biosensor (BSA or gelatin) placed on a quartz microbalance on a chip (LoC: lab on a chip) was able to measure accurately different kinds of tannins. Further proteins are being tested. © 2023 International Society for Horticultural Science. All rights reserved.
Development of a micro-Vis-NIR and SAW nanobiosensor to measure polyphenols in must/wine on-time and online
Cecchini M;Gagliardi M
2023
Abstract
The need for measuring polyphenols during the vinification process is an old story. Today the most up-to-date non-destructive instrument for wine and must used in wineries is the FOSS, Winescan, based on FT-IR technology. But wineries need something which measures polyphenols during the extraction in order to automatize the process. We have followed two lines of research: one is to develop a low cost micro-NIR instrument and the other the development of a biosensor based on protein binding to polyphenol. In the first case, a homemade, low cost, Vis-NIR micro sensor on Arduino platform was used. Spectra have been acquired on different grape must cultivars, wines and chemometrics, to elaborate the correlation of spectra with destructive analyses, revealed an acceptable level of confidence but still not completely reliable. The other research is based on the application of SAW (surface acoustic waves) with selected protein to bind polyphenols. The SAW resonator biosensor (BSA or gelatin) placed on a quartz microbalance on a chip (LoC: lab on a chip) was able to measure accurately different kinds of tannins. Further proteins are being tested. © 2023 International Society for Horticultural Science. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
