The nano-LC technique is increasingly used for both fast studies on enantiomericanalysis and test beds of novel stationary phases due to the small volumesinvolved and the short conditioning and analysis times. In this study, the enantioseparation of 10 drugs from different families was carried out by nano-LC, utilizing silica with immobilized amylose tris(3-chloro-5-methylphenylcarbamate)column. The effect on chiral separation caused by the addition of different saltsto the mobile phase was evaluated. To simultaneously separate as many enantiomers as possible, the effect of buffer concentration in the mobile phase wasstudied, and, to increase the sensitivity, a liquid-liquid microextraction basedon the use of isoamyl acetate as sustainable extraction solvent was applied topre-concentrate four chiral drugs from tap and environmental waters, achievingsatisfactory recoveries (>70%).
Enantiomeric analysis of drugs in water samples by using liquid-liquid microextraction and nano-liquid chromatography.
Salvatore Fanali
2023
Abstract
The nano-LC technique is increasingly used for both fast studies on enantiomericanalysis and test beds of novel stationary phases due to the small volumesinvolved and the short conditioning and analysis times. In this study, the enantioseparation of 10 drugs from different families was carried out by nano-LC, utilizing silica with immobilized amylose tris(3-chloro-5-methylphenylcarbamate)column. The effect on chiral separation caused by the addition of different saltsto the mobile phase was evaluated. To simultaneously separate as many enantiomers as possible, the effect of buffer concentration in the mobile phase wasstudied, and, to increase the sensitivity, a liquid-liquid microextraction basedon the use of isoamyl acetate as sustainable extraction solvent was applied topre-concentrate four chiral drugs from tap and environmental waters, achievingsatisfactory recoveries (>70%).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
