The soil microfungi that release biosurfactants and tolerate recalcitrant organic pollutants such as polycyclic aromatic hydrocarbons (PAHs) may be of outstanding importance for mycoremediation of polluted sites, in a cost-efficient and environmentally friendly manner. Minimedusa polyspora FBL 503 (Agaricomycetes, Cantharellales) showed characteristics with a high biotech potential. The strain FBL 503 tolerated naphthalene, phenanthrene, pyrene, each PAH at 20 mg/L, and a mix of them (1:1:1) at 60 mg/L as well as exhibited biosurfactant activity. In particular, the production of biosurfactants was carried out in yeast extract broth (10g/L) with the addition of 40g/L of olive oil. Fourteen days after inoculation, the culture medium was recovered and the production of biosurfactants was evaluated by means of the oil dispersion test. The activity of the biosurfactants found in the culture filtrate was evaluated on olive oil, diesel oil, and exhausted motor oils from a scooter and a freeze-dryer vacuum pump. The biosurfactants from FBL 503 resulted efficient, to different extent, in displacing all the tested oils. In this context, we obtained the draft genome sequence of the strain FBL 503 with Illumina platform. The genome was assembled denovo and was used to functionally annotate M. polyspora FBL 503. We calculated the predicted genes, including genes encoding secreted CAZymes, proteases, and lipases present in the genome. Moreover, mining the genome for genes involved in secondary metabolism biosynthesis resulted in the identification of biosynthetic gene clusters. The genes encoding enzymes for the enhanced solubility of PAHs such as biosurfactants and lipases, which could be included in the PAH degradation pathway, were found in the M. polyspora FBL 503 genome. Our genomic data on FBL 503 are a basis for transcriptomic approaches under exposure to organic pollutants. The INAIL-DIT (PAR 2019-2021) financially supported the genome sequencing of M. polyspora FBL 503.
Draft genome sequence of the biosurfactant-producing Minimedusa polyspora FBL 503 (Basidiomycota; Agaricomycotina)
Pinzari Flavia;
2023
Abstract
The soil microfungi that release biosurfactants and tolerate recalcitrant organic pollutants such as polycyclic aromatic hydrocarbons (PAHs) may be of outstanding importance for mycoremediation of polluted sites, in a cost-efficient and environmentally friendly manner. Minimedusa polyspora FBL 503 (Agaricomycetes, Cantharellales) showed characteristics with a high biotech potential. The strain FBL 503 tolerated naphthalene, phenanthrene, pyrene, each PAH at 20 mg/L, and a mix of them (1:1:1) at 60 mg/L as well as exhibited biosurfactant activity. In particular, the production of biosurfactants was carried out in yeast extract broth (10g/L) with the addition of 40g/L of olive oil. Fourteen days after inoculation, the culture medium was recovered and the production of biosurfactants was evaluated by means of the oil dispersion test. The activity of the biosurfactants found in the culture filtrate was evaluated on olive oil, diesel oil, and exhausted motor oils from a scooter and a freeze-dryer vacuum pump. The biosurfactants from FBL 503 resulted efficient, to different extent, in displacing all the tested oils. In this context, we obtained the draft genome sequence of the strain FBL 503 with Illumina platform. The genome was assembled denovo and was used to functionally annotate M. polyspora FBL 503. We calculated the predicted genes, including genes encoding secreted CAZymes, proteases, and lipases present in the genome. Moreover, mining the genome for genes involved in secondary metabolism biosynthesis resulted in the identification of biosynthetic gene clusters. The genes encoding enzymes for the enhanced solubility of PAHs such as biosurfactants and lipases, which could be included in the PAH degradation pathway, were found in the M. polyspora FBL 503 genome. Our genomic data on FBL 503 are a basis for transcriptomic approaches under exposure to organic pollutants. The INAIL-DIT (PAR 2019-2021) financially supported the genome sequencing of M. polyspora FBL 503.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
