Timely and noninvasive detection of plant diseases may help to mitigate significant yield losses and represents animportant milestone in sustainable plant protection. Herein, we report the application of pulsed thermography (PT) to predictpresymptomatic stages of gray mold. For this purpose, pepper and tomato plants were inoculated with conidia of Botrytis cinerea orTrichoderma harzianum, a beneficial fungus employed as nonpathogenic control. Time-course measurements on infected leavesshowed that, unlike classical passive thermography, PT revealed thermal patterns within a few hours after inoculation, much earlierthan the appearance of necrotic lesions caused by B. cinerea. Diagnostic parameters together with physiological and molecularanalyses confirmed a high reliability of PT in the early detection of B. cinerea infection. Collectively, our data demonstrate that PTimaging is a valid and reliable tool for the early and noninvasive detection of B. cinerea infection and opens new paths for sustainableand precise agriculture.

Pulsed Thermography as a Reliable Tool to Detect Presymptomatic Stages of Botrytis cinerea Infection in Plants

Ruocco M;
2023

Abstract

Timely and noninvasive detection of plant diseases may help to mitigate significant yield losses and represents animportant milestone in sustainable plant protection. Herein, we report the application of pulsed thermography (PT) to predictpresymptomatic stages of gray mold. For this purpose, pepper and tomato plants were inoculated with conidia of Botrytis cinerea orTrichoderma harzianum, a beneficial fungus employed as nonpathogenic control. Time-course measurements on infected leavesshowed that, unlike classical passive thermography, PT revealed thermal patterns within a few hours after inoculation, much earlierthan the appearance of necrotic lesions caused by B. cinerea. Diagnostic parameters together with physiological and molecularanalyses confirmed a high reliability of PT in the early detection of B. cinerea infection. Collectively, our data demonstrate that PTimaging is a valid and reliable tool for the early and noninvasive detection of B. cinerea infection and opens new paths for sustainableand precise agriculture.
2023
Istituto per la Protezione Sostenibile delle Piante - IPSP - Sede Secondaria Portici (NA)
pulsed thermography
presymtomatic disease detection
Botrytis cinerea
gray mold
plant-pathogen interactions
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.14243/458464
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