Animal models represent the gold standard for toxicity assessment. However, due to anatomical and functional diversity, they are often unable to replicate the real human physio-pathological scenario with sufficient fidelity. Additionally, their use is currently strongly debated due to ethical issues. In this scenario, the development of in vitro bioengineered tissue/organ models could represent a significant step forward in establishing Novel Approach Methodologies, providing more reliable and robust methods for toxicity assessment [1]. In this contribution, we report the development of a new in vitro bioengineered model of cardiac tissue. The model results from the assembly of a polymeric 3D multi-layered structure obtained by melt extrusion additive manufacturing of a custom-made poly(ester urethane) (PU) with a cell-loaded (human induced pluripotent stem cell derived-cardiomyocytes hiPSC-CMs) methacryloyl gelatin bio-ink.
Engineering 3D in vitro cardiac tissue models for cardiotoxicity assessment
Vozzi F;Gisone I;Cecchettini A;Guiducci L;Del Ry S;Cabiati M;
2022
Abstract
Animal models represent the gold standard for toxicity assessment. However, due to anatomical and functional diversity, they are often unable to replicate the real human physio-pathological scenario with sufficient fidelity. Additionally, their use is currently strongly debated due to ethical issues. In this scenario, the development of in vitro bioengineered tissue/organ models could represent a significant step forward in establishing Novel Approach Methodologies, providing more reliable and robust methods for toxicity assessment [1]. In this contribution, we report the development of a new in vitro bioengineered model of cardiac tissue. The model results from the assembly of a polymeric 3D multi-layered structure obtained by melt extrusion additive manufacturing of a custom-made poly(ester urethane) (PU) with a cell-loaded (human induced pluripotent stem cell derived-cardiomyocytes hiPSC-CMs) methacryloyl gelatin bio-ink.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.