During a nematode survey, severe infections of tobacco feeder roots and heavy soil infestations by Meloidogyne incognita race 1 were found in S. Miguel (Azores islands, Portugal). This is thought to be the first record of M. incognita infection of tobacco in Azores. The morphology of various life stages, analysis of the esterase electrophoretic pattern and differential host tests were used for nematode characterization and identification. Nematode-induced mature galls were spherical and/or ellipsoidal and usually contained more than one female, males and egg masses with eggs. Feeding sites were characterized by the development of giant cells that contained granular cytoplasm and many hypertrophied nuclei. Giant cell cytoplasm was aggregated along a thickened cell wall. Vascular tissues within galls appeared disorganized. The relationship between the initial nematode population density and growth of tobacco plants was tested in a glasshouse experiment in which inoculum levels varied from 0 to 512 eggs and juveniles (J2) cm-3 of the soil. Seinhorst's model was fitted to height and top fresh weight data of the inoculated and control plants. Tolerance limits with respect to plant height and fresh top weight of tobacco cv. Erzegovina plants to M. incognita race 1 were estimated as 1.25 eggs and J2 cm-3 of the soil. The maximum nematode reproduction rate was 404.7 at an initial population density of 4 eggs and J2 cm-3 of the soil.
Host parasitic relationships in tobacco plants infected with a root-knot nematode (Meloidogyne incognita) population from the Azores.
Vovlas N;Sasanelli N;
2004
Abstract
During a nematode survey, severe infections of tobacco feeder roots and heavy soil infestations by Meloidogyne incognita race 1 were found in S. Miguel (Azores islands, Portugal). This is thought to be the first record of M. incognita infection of tobacco in Azores. The morphology of various life stages, analysis of the esterase electrophoretic pattern and differential host tests were used for nematode characterization and identification. Nematode-induced mature galls were spherical and/or ellipsoidal and usually contained more than one female, males and egg masses with eggs. Feeding sites were characterized by the development of giant cells that contained granular cytoplasm and many hypertrophied nuclei. Giant cell cytoplasm was aggregated along a thickened cell wall. Vascular tissues within galls appeared disorganized. The relationship between the initial nematode population density and growth of tobacco plants was tested in a glasshouse experiment in which inoculum levels varied from 0 to 512 eggs and juveniles (J2) cm-3 of the soil. Seinhorst's model was fitted to height and top fresh weight data of the inoculated and control plants. Tolerance limits with respect to plant height and fresh top weight of tobacco cv. Erzegovina plants to M. incognita race 1 were estimated as 1.25 eggs and J2 cm-3 of the soil. The maximum nematode reproduction rate was 404.7 at an initial population density of 4 eggs and J2 cm-3 of the soil.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
