cDNA arrays were used to explore mechanisms controlling fruiting body development in the truffle Tuber borchii. Differences in gene expression were higher between reproductive and vegetative stage than between two stages of fruiting body maturation. We suggest hypotheses about the importance of various physiological processes during the development of fruiting bodies. Irrespective of their nutritional strategies, most saprotrophic and mycorrhizal fungi produce conspicuous fruiting bodies where hyphae aggregate, produce pseudotissues with differentiated compartments, develop specialized structures, and eventually differentiate meiotic spores. Among them, the ectomycorrhizal truffles (Tuber spp.) produce hypogeous ascocarps which are highly appreciated and commercialized for their delicate organoleptic properties. Since truffle fruiting bodies cannot yet be obtained under controlled conditions, our knowledge of the morphogenetic events leading to ascocarp development and maturation, as well as their underlying molecular bases, is quite limited. Elucidating the spatiotemporal control of gene expression during the successive stages of the truffle life cycle will improve our knowledge of processes that initiate and coordinate the formation of hypogeous truffles. Here, we describe changes in gene expression during the formation of the ascomata of Tuber borchii. Unripe (CF05; 0 to 5% mature spores) and ripe (CF70; 70 to 100% mature spores) T. borchii fruiting bodies were collected under hazelnut trees from a natural truffle ground near Alba in Piedmont (Italy) during the 2000 to 2001 production seasons. RNA was extracted as described by Lacourt et al. cDNA libraries were constructed using the PCR-based SMART cDNA library construction kit in LambdaTriplEx2 (Clontech, Palo Alto, CA). A cDNA array containing 2,041 elements was produced, hybridized, and analyzed according to Duplessis et al. Six cDNA complex probes were then prepared from total RNA of two CF05 ascomata, two CF70 ascomata, and vegetative mycelium. During fruiting body development, the vast majority of genes were not significantly regulated among the different stages. However, comparisons between fruiting bodies and mycelium indicated that 69 nonredundant transcripts (i.e., 3%) showed significant changes in expression (analysis of variance, P < 0.01) (Table 1). In addition, inferences were only made from genes showing a differential expression ratio above 2.5 (below 0.4) between any two stages.
Transcript profiling reveals novel marker genes involved in fruiting body formation in Tuber borchii.
Abbà S;Bonfante P
2005
Abstract
cDNA arrays were used to explore mechanisms controlling fruiting body development in the truffle Tuber borchii. Differences in gene expression were higher between reproductive and vegetative stage than between two stages of fruiting body maturation. We suggest hypotheses about the importance of various physiological processes during the development of fruiting bodies. Irrespective of their nutritional strategies, most saprotrophic and mycorrhizal fungi produce conspicuous fruiting bodies where hyphae aggregate, produce pseudotissues with differentiated compartments, develop specialized structures, and eventually differentiate meiotic spores. Among them, the ectomycorrhizal truffles (Tuber spp.) produce hypogeous ascocarps which are highly appreciated and commercialized for their delicate organoleptic properties. Since truffle fruiting bodies cannot yet be obtained under controlled conditions, our knowledge of the morphogenetic events leading to ascocarp development and maturation, as well as their underlying molecular bases, is quite limited. Elucidating the spatiotemporal control of gene expression during the successive stages of the truffle life cycle will improve our knowledge of processes that initiate and coordinate the formation of hypogeous truffles. Here, we describe changes in gene expression during the formation of the ascomata of Tuber borchii. Unripe (CF05; 0 to 5% mature spores) and ripe (CF70; 70 to 100% mature spores) T. borchii fruiting bodies were collected under hazelnut trees from a natural truffle ground near Alba in Piedmont (Italy) during the 2000 to 2001 production seasons. RNA was extracted as described by Lacourt et al. cDNA libraries were constructed using the PCR-based SMART cDNA library construction kit in LambdaTriplEx2 (Clontech, Palo Alto, CA). A cDNA array containing 2,041 elements was produced, hybridized, and analyzed according to Duplessis et al. Six cDNA complex probes were then prepared from total RNA of two CF05 ascomata, two CF70 ascomata, and vegetative mycelium. During fruiting body development, the vast majority of genes were not significantly regulated among the different stages. However, comparisons between fruiting bodies and mycelium indicated that 69 nonredundant transcripts (i.e., 3%) showed significant changes in expression (analysis of variance, P < 0.01) (Table 1). In addition, inferences were only made from genes showing a differential expression ratio above 2.5 (below 0.4) between any two stages.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
