In this study, we describe a 2D-SERS sensor obtained by deposition of spherical gold nanoparticles (AuNPs) onto a suitably functionalized metal surface. Morphological analysis of the SERS surface by SEM and AFM demonstrated a uniform and stable distribution of the active nanoparticles. Following p-mercaptoaniline (pMA) functionalization, the sensor was characterized by co-localized Raman measurements, demonstrating a significant enhancement in Raman signals with homogeneous SERS activity across the entire sampled area. The as-prepared SERS sensor was demonstrated to be suitable for Therapeutic Drug Monitoring (TDM) of 6-mercaptopurine (6- MP), exhibiting a linear correlation between analyte concentration and SERS intensity in the range 5 – 20 μM. This work highlights the potential of 2D-SERS sensors for hypersensitive and accurate analytical measurements, particularly in the biomedical field.

A stable and sensitive 2D SERS sensor for bioanalytical applications

Pannico, Marianna
Primo
;
Musto, Pellegrino
Ultimo
2024

Abstract

In this study, we describe a 2D-SERS sensor obtained by deposition of spherical gold nanoparticles (AuNPs) onto a suitably functionalized metal surface. Morphological analysis of the SERS surface by SEM and AFM demonstrated a uniform and stable distribution of the active nanoparticles. Following p-mercaptoaniline (pMA) functionalization, the sensor was characterized by co-localized Raman measurements, demonstrating a significant enhancement in Raman signals with homogeneous SERS activity across the entire sampled area. The as-prepared SERS sensor was demonstrated to be suitable for Therapeutic Drug Monitoring (TDM) of 6-mercaptopurine (6- MP), exhibiting a linear correlation between analyte concentration and SERS intensity in the range 5 – 20 μM. This work highlights the potential of 2D-SERS sensors for hypersensitive and accurate analytical measurements, particularly in the biomedical field.
2024
Istituto per i Polimeri, Compositi e Biomateriali - IPCB
SERS, Raman imaging, AFM, Therapeutic Drug Monitoring, 6-mercaptopurine
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.14243/495241
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