Anthocyanins are naturally occurring secondary metabolites, responsible for the red/purple pigmentation of many plant organs. At the transcriptional level, anthocyanin biosynthesis is mainly regulated by well-characterized bHLH and MYB transcription factors (TFs). We produced tomato plants (cv. Micro-Tom) ectopically expressing the Arabidopsis AtMYB90/ Production of Anthocyanin Pigment2 (PAP2) gene under the control of the constitutive CaMV35S promoter. Three independent 35S:MYB90 lines (L1, L2, and L3) disclosing high levels of transgene expression were selected for further analyses. At the seedling stage, the transgenic lines revealed intense pigmentation of leaves and petioles.
ECTOPIC EXPRESSION OF ATMYB90 IN TOMATO RESULTS IN INCREASED ANTHOCYANIN ACCUMULATION AND DEFECTIVE POLLEN DEVELOPMENT
BALDONI E.;COMINELLI E.;LOCATELLI F.;SPARVOLI F.;GALBIATI M.
2023
Abstract
Anthocyanins are naturally occurring secondary metabolites, responsible for the red/purple pigmentation of many plant organs. At the transcriptional level, anthocyanin biosynthesis is mainly regulated by well-characterized bHLH and MYB transcription factors (TFs). We produced tomato plants (cv. Micro-Tom) ectopically expressing the Arabidopsis AtMYB90/ Production of Anthocyanin Pigment2 (PAP2) gene under the control of the constitutive CaMV35S promoter. Three independent 35S:MYB90 lines (L1, L2, and L3) disclosing high levels of transgene expression were selected for further analyses. At the seedling stage, the transgenic lines revealed intense pigmentation of leaves and petioles.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
