Pleiotropic drug resistant (PDR/ABCG) genes are involved in plant response to biotic and abiotic stresses. In this work, we cloned, from Solanum tuberosum, four PDR/ABCG transporter genes named StPDR1, StPDR2, StPDR3 and StPDR4, which were differentially expressed in plant tissues and cell cultures. A number of different chemically unrelated compounds were found to regulate the transcript levels of the four genes in cultured cells. In particular, StPDR2 was highly up-regulated in the presence of Botrytis cinerea cell walls, NaCl, 2,4-dichlorophenol, sclareol and a-solanin and biological compounds. The expression of the genes was also investigated by real time RT-PCR during infection by Phytophthora infestans. StPDR1 and StPDR2 were up-regulated about 13- and 37-fold at 48 h post-infection (hpi), StPDR3 was expressed (45-fold) at 24 and 48 hpi and then rapidly decreased, while StPDR4 RNA accumulation was stimulated (about 4-fold)at 12 and 24 hpi, decreased at 48 hpi and increased again at 96 hpi. We discuss the role of StPDR14 genes in response to pathogens and abiotic stresses.
Four potato (Solanum tuberosum) ABCG transporters and their expression in response to abiotic factors and Phytophthora infestans infection
Ruocco M;
2011
Abstract
Pleiotropic drug resistant (PDR/ABCG) genes are involved in plant response to biotic and abiotic stresses. In this work, we cloned, from Solanum tuberosum, four PDR/ABCG transporter genes named StPDR1, StPDR2, StPDR3 and StPDR4, which were differentially expressed in plant tissues and cell cultures. A number of different chemically unrelated compounds were found to regulate the transcript levels of the four genes in cultured cells. In particular, StPDR2 was highly up-regulated in the presence of Botrytis cinerea cell walls, NaCl, 2,4-dichlorophenol, sclareol and a-solanin and biological compounds. The expression of the genes was also investigated by real time RT-PCR during infection by Phytophthora infestans. StPDR1 and StPDR2 were up-regulated about 13- and 37-fold at 48 h post-infection (hpi), StPDR3 was expressed (45-fold) at 24 and 48 hpi and then rapidly decreased, while StPDR4 RNA accumulation was stimulated (about 4-fold)at 12 and 24 hpi, decreased at 48 hpi and increased again at 96 hpi. We discuss the role of StPDR14 genes in response to pathogens and abiotic stresses.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
