: Microglia reactivity entails a large-scale remodeling of cellular geometry, but the behavior of the microtubule cytoskeleton during these changes remains unexplored. Here we show that activated microglia provide an example of microtubule reorganization from a non-centrosomal array of parallel and stable microtubules to a radial array of more dynamic microtubules. While in the homeostatic state, microglia nucleate microtubules at Golgi outposts, and activating signaling induces recruitment of nucleating material nearby the centrosome, a process inhibited by microtubule stabilization. Our results demonstrate that a hallmark of microglia reactivity is a striking remodeling of the microtubule cytoskeleton and suggest that while pericentrosomal microtubule nucleation may serve as a distinct marker of microglia activation, inhibition of microtubule dynamics may provide a different strategy to reduce microglia reactivity in inflammatory disease.

Microglia reactivity entails microtubule remodeling from acentrosomal to centrosomal arrays

Gosti, Giorgio;Catalano, Federico;Indrieri, Alessia;De Leonibus, Elvira;Ruocco, Giancarlo;
2023

Abstract

: Microglia reactivity entails a large-scale remodeling of cellular geometry, but the behavior of the microtubule cytoskeleton during these changes remains unexplored. Here we show that activated microglia provide an example of microtubule reorganization from a non-centrosomal array of parallel and stable microtubules to a radial array of more dynamic microtubules. While in the homeostatic state, microglia nucleate microtubules at Golgi outposts, and activating signaling induces recruitment of nucleating material nearby the centrosome, a process inhibited by microtubule stabilization. Our results demonstrate that a hallmark of microglia reactivity is a striking remodeling of the microtubule cytoskeleton and suggest that while pericentrosomal microtubule nucleation may serve as a distinct marker of microglia activation, inhibition of microtubule dynamics may provide a different strategy to reduce microglia reactivity in inflammatory disease.
2023
Istituto di Biochimica e Biologia Cellulare - IBBC - Sede Secondaria Monterotondo
Istituto di Scienze del Patrimonio Culturale - ISPC - Sede Secondaria Roma
Istituto di Ricerca Genetica e Biomedica - IRGB
Istituto di Bioscienze e Biorisorse - IBBR - Sede Secondaria Napoli
CP: Molecular biology
CP: Neuroscience
Golgi outposts
MTOC
activated microglia
alternatively activated microglia
centrosome
inflammation
microtubule
reactive microglia
retina
γ-tubulin
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.14243/514228
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