Phytochemical analysis for the residues of Opuntia ficus indica l seed oil of eastern region of Morocco

The cactus pear fruit (Opuntia ficus-indica L. Mill.) is a product that has been recognized for the health benefits that it can provide, and it has also drawn scientific interest for several years. The objective of this study is to identify chemically the residues (Cakes oil) of Opuntia ficus indica l seed oil of eastern region of Morocco. A mechanical extraction is used to obtained this oil residues then we used maceration for the analysis extraction. Various phytochemical assays were used to determine the qualitative chemical composition, which was subsequently characterized using GC–MS and HPLC-DAD, LC-MS/MS. According to GC–MS analyses, which allow the identification of tree major fatty acids, (linoleic acid, oleic acid and palmitic acid), linoleic acid is abundant (37.218 %) for OFI seeds oil on the contrary, oleic acid is abundant (27.169 %) for OFI. HPLC-DAD analysis allows the identification of tocopherols, with the γ-tocopherols is the major tocopherol for with 310.01 ± 3.33 mg/Kg, the LC-MS/MS allow the identification of polyphenols and flavonoids, Among phenolic acids, residues of OFI was marked by the predominant of syringic acid (18.33 %), Arbutin (14.46 %), oleuropein (11.50 %) and p coumaric acid with (7.10 %).