A Pseudomonas aeruginosa-Suitable Fluorescent Reporter System for Analyzing Small RNA-Mediated Regulation of Target mRNAs

: Bacterial small RNAs have emerged as modulators that play key roles in regulatory networks related to viability, environmental adaptation, and pathogenesis. Small RNAs can modulate gene expression by base-pairing to target mRNAs, influencing their translation and/or stability. A superfolder GFP reporter system, previously developed for Escherichia coli and Salmonella enterica, was adapted to Pseudomonas aeruginosa and used to validate novel mRNA targets in studies of small RNA-mediated regulatory mechanisms.