This review aims to highlight the most recent applications of CZE- MS in separation and characterization of mAb biotherapeutics based on the most recent literature (2022-2025). The various analytical approaches used in proteins MS characterization will be illustrated, with particular emphasis on the top-down approach, widely applied in this field of analysis and offering comprehensive characterization of proteoforms and exploration of protein interactions under native conditions. CZE-MS demonstrated an effective analytical strategy for the detailed characterization of mAb proteoforms with high potential for application in the challenging task of monitoring mAb release in advanced drug delivery systems.
The challenges and prospects of capillary zone electrophoresis-mass spectrometry for characterization of monoclonal antibodies biotherapeutics
C. Desiderio;
2026
Abstract
This review aims to highlight the most recent applications of CZE- MS in separation and characterization of mAb biotherapeutics based on the most recent literature (2022-2025). The various analytical approaches used in proteins MS characterization will be illustrated, with particular emphasis on the top-down approach, widely applied in this field of analysis and offering comprehensive characterization of proteoforms and exploration of protein interactions under native conditions. CZE-MS demonstrated an effective analytical strategy for the detailed characterization of mAb proteoforms with high potential for application in the challenging task of monitoring mAb release in advanced drug delivery systems.| File | Dimensione | Formato | |
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