Characterization of Different Soil Bacterial Strains and Assessment of Their Impact on the Growth of Triticum turgidum spp. durum and Lens culinaris spp. culinaris

Plant growth-promoting bacteria (PGPB) are vital for enhancing plant growth, productivity, and sustainability in agriculture, also addressing food security challenges. The plant growth-promoting (PGP) potential of ten bacterial strains, isolated from a cultivated field in southern Italy, was characterized with biochemical and molecular analyses and plant growth-promoting activity was tested on two durum wheat varieties (RGT Aventadur and Farah) and a lentil one (Altamura Lentil) under semi-controlled conditions. The isolated strains were classified using 16S rRNA gene sequencing. Results showed that they belonged to Pseudomonaceae, Rhizobiaceae, Bacillaceae and Micrococcaceae families. They exhibited typical features of PGPB, such as inorganic phosphate solubilization, production of indole acetic acid, ammonia, and biofilm formation. Bacterial inoculation of wheat plants led to the identification of potentially interesting strains that positively affected biometric parameters (i.e., shoot height, tiller number and spike weight) in a genotype-dependent way. The contrasting effect of some bacterial strains on the two wheat genotypes supports the necessity to accurately formulate synthetic microbial consortia characterized by long-term PGP traits, taking into account that the application under field conditions might also be influenced by native soil microbiota.