The research here reported deals with the activity of treatments against pathogens associated with esca on young vines, and their efficacy for the reduction of foliar symptom expression. Copper oxychloride plus gentian violet (Labimethyl®) is a peptide complex copper formulation that appears to be able to enter the plant tissue. Laboratory trials were carried out as following: 1) the growth rate inhibition of the formulate was tested in vitro on Phaeomoniella chlamydospora (Pch), Phaeoacremonium aleophilum (Pal) and Fomitiporia mediterranea (Fmed); 2) the Pch and Pal colonies were grown in liquid medium containing the formulate and cultural filtrates were analyzed for the presence of the phytotoxin scytalone; 3) the amount of copper penetrated inside the trunk of treated potted vines was analyzed by Atomic Absorption Spectrophotometer. Further trials were conducted on potted vines inoculated with Pch, and in vineyards naturally esca infected. The formulate was sprayed onto the trunk of potted vines or in the vineyard soon after grape harvesting, at the end of pruning, and at 6-7 cm shoot length as recommended in the experimental plan proposed by the manufacturer. The formulate was effective in vitro for Pch, Pal and Fmed giving EC50 values of 0.4, 3.31 and 5.53 mg copper L-1 respectively; scytalone was not found in the culture filtrate of Pch and Pal grown in the presence of the formulate (detection sensitivity 2 ppm); copper was found inside the trunk of potted vines. Treatments on inoculated potted plants did not give significant reductions of the necrosis length caused by Pch, while in the vineyard a reduction of foliar symptom expression was assessed in treated plots.

Effects of a copper oxychloride formulation against esca and associated fungi

DI MARCO S;OSTI F;
2010

Abstract

The research here reported deals with the activity of treatments against pathogens associated with esca on young vines, and their efficacy for the reduction of foliar symptom expression. Copper oxychloride plus gentian violet (Labimethyl®) is a peptide complex copper formulation that appears to be able to enter the plant tissue. Laboratory trials were carried out as following: 1) the growth rate inhibition of the formulate was tested in vitro on Phaeomoniella chlamydospora (Pch), Phaeoacremonium aleophilum (Pal) and Fomitiporia mediterranea (Fmed); 2) the Pch and Pal colonies were grown in liquid medium containing the formulate and cultural filtrates were analyzed for the presence of the phytotoxin scytalone; 3) the amount of copper penetrated inside the trunk of treated potted vines was analyzed by Atomic Absorption Spectrophotometer. Further trials were conducted on potted vines inoculated with Pch, and in vineyards naturally esca infected. The formulate was sprayed onto the trunk of potted vines or in the vineyard soon after grape harvesting, at the end of pruning, and at 6-7 cm shoot length as recommended in the experimental plan proposed by the manufacturer. The formulate was effective in vitro for Pch, Pal and Fmed giving EC50 values of 0.4, 3.31 and 5.53 mg copper L-1 respectively; scytalone was not found in the culture filtrate of Pch and Pal grown in the presence of the formulate (detection sensitivity 2 ppm); copper was found inside the trunk of potted vines. Treatments on inoculated potted plants did not give significant reductions of the necrosis length caused by Pch, while in the vineyard a reduction of foliar symptom expression was assessed in treated plots.
2010
Istituto di Biometeorologia - IBIMET - Sede Firenze
Phaeoacremonium aleophilum
Phaeomoniella chlamydospora
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.14243/61531
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