In the grain legume Cicer arietinum L. (chickpea), the seed-derived embryo axes deprived of the apical meristem were able to regenerate adventitious shoots on Murashige and Skoog (1962) medium supplemented with kinetin. This protocol was suitable for Agrobacterium-mediated gene transfer by the co-cultivation technique. Chickpea transgenic plants showed neomycin phosphotransferase Il and beta-glucuronidase activities and the presence in their genome of integrated bacterial DNA.
Genetic transformation in the grain legume Cicer arietinum L.(chickpea)
S Caretto;G Frugis;
1993
Abstract
In the grain legume Cicer arietinum L. (chickpea), the seed-derived embryo axes deprived of the apical meristem were able to regenerate adventitious shoots on Murashige and Skoog (1962) medium supplemented with kinetin. This protocol was suitable for Agrobacterium-mediated gene transfer by the co-cultivation technique. Chickpea transgenic plants showed neomycin phosphotransferase Il and beta-glucuronidase activities and the presence in their genome of integrated bacterial DNA.File in questo prodotto:
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