Three complementary collections of the model species Medicago truncatula (Porceddu et al., 2008) were produced in the frame of the Italian functional genomics project MIUR-FIRB 'Post-genomics of Forage Legumes'. A wide range of variation in plant and plant organ morphology was found in the TILLING collection formed by about 1900 lines with DNA (M2 generation) and seed (M3/M4 generation). The estimated rate of mutation, on the basis of the screening for three genes of interest, was 1 mutation/Kbp/400 plants. Two of these genes are involved in the biosynthetic pathway of plant secondary metabolites (triterpenic saponins and trypsin inhibitors) displaying physiological, agronomical and industrial interest. In particular, two segregant mutant lines of the 6 found for the orthologue of the trypsin inhibitor gene MsTI from M. scutellata were examined but no significant variation in trypsin inhibition was found compared to the full sib wild type. Two lines carrying different point mutations in the MtPHY1 gene, coding for an extracellular phytase, were also studied, one of them showing different (P = 7.5%) enhanced phytase activity with respect to the control line. Transposon mutagenized lines were also produced (approx. 1500 R0 plants) according to the strategy of d'Erfurth et al. (2003). Several mutants were isolated also regarding plant architecture (for example leaf and flower morphology) and secondary compounds (tannins). Results will be presented on few selected mutants that have been characterized more extensively.
COLLECTIONS OF MUTANTS FOR FUNCTIONAL GENOMICS IN THE LEGUME MEDICAGO TRUNCATULA
CALDERINI O;ARCIONI S
2010
Abstract
Three complementary collections of the model species Medicago truncatula (Porceddu et al., 2008) were produced in the frame of the Italian functional genomics project MIUR-FIRB 'Post-genomics of Forage Legumes'. A wide range of variation in plant and plant organ morphology was found in the TILLING collection formed by about 1900 lines with DNA (M2 generation) and seed (M3/M4 generation). The estimated rate of mutation, on the basis of the screening for three genes of interest, was 1 mutation/Kbp/400 plants. Two of these genes are involved in the biosynthetic pathway of plant secondary metabolites (triterpenic saponins and trypsin inhibitors) displaying physiological, agronomical and industrial interest. In particular, two segregant mutant lines of the 6 found for the orthologue of the trypsin inhibitor gene MsTI from M. scutellata were examined but no significant variation in trypsin inhibition was found compared to the full sib wild type. Two lines carrying different point mutations in the MtPHY1 gene, coding for an extracellular phytase, were also studied, one of them showing different (P = 7.5%) enhanced phytase activity with respect to the control line. Transposon mutagenized lines were also produced (approx. 1500 R0 plants) according to the strategy of d'Erfurth et al. (2003). Several mutants were isolated also regarding plant architecture (for example leaf and flower morphology) and secondary compounds (tannins). Results will be presented on few selected mutants that have been characterized more extensively.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
