Dills (Anethum graveolens L.) grown from seeds in a small area of the Italian Riviera showed symptoms including stalk discoloration, flower virescence and phyllody. The affected plants were tested for the presence of phytoplasmas. DNAs were PCR-amplified in reactions with ribosomal universal or group-specific primer pairs and analysed by restriction digestions with BfaI or MseI enzyme. Phytoplasmas, identified as members of 16SrI-B, I-C, V-A and XII-A subgroups were present in 18 out of 22 samples (81.8%). Of these, 9 (50% of the infected) carried a single phytoplasma [3 subgroup 16SrI-B (16.6%), 1 16SrI-C (5.5%), and 5 16SrV-A (27.7%)]. Seven samples (38.8%) carried two different phytoplasmas (6 members of subgroups V-A and XII-A; I member of subgroups I-C and V-A). Two samples carried phytoplasmas of three different subgroups in different mixes (I-C, V-A and XII-A; I-B,I-C and XII-A). There was no clear correlation between a given symptom and the presence of one or more phytoplasmas. This is the first report from Europe of phytoplasmas in dill plants.
molecular identification of phytoplasmas from Dill (Anethum Graveolens L.; Umbelliferae).
2002
Abstract
Dills (Anethum graveolens L.) grown from seeds in a small area of the Italian Riviera showed symptoms including stalk discoloration, flower virescence and phyllody. The affected plants were tested for the presence of phytoplasmas. DNAs were PCR-amplified in reactions with ribosomal universal or group-specific primer pairs and analysed by restriction digestions with BfaI or MseI enzyme. Phytoplasmas, identified as members of 16SrI-B, I-C, V-A and XII-A subgroups were present in 18 out of 22 samples (81.8%). Of these, 9 (50% of the infected) carried a single phytoplasma [3 subgroup 16SrI-B (16.6%), 1 16SrI-C (5.5%), and 5 16SrV-A (27.7%)]. Seven samples (38.8%) carried two different phytoplasmas (6 members of subgroups V-A and XII-A; I member of subgroups I-C and V-A). Two samples carried phytoplasmas of three different subgroups in different mixes (I-C, V-A and XII-A; I-B,I-C and XII-A). There was no clear correlation between a given symptom and the presence of one or more phytoplasmas. This is the first report from Europe of phytoplasmas in dill plants.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.