Structure and evolution of the 7SL RNA component of the Signal Recognition Particle

We have cloned and characterized cDNA copies of larval and adult Drosophila 7SL RNA. The Drosophila 7SL sequence shares 66.3% homology with that of human 7SL RNA. The homology is not evenly distributed along the sequence, but is concentrated in blocks in the central part of the molecule. We have analysed the secondary stmcture of Drosophila and human 7SL RNA free in solution by digestion with single and double strand specific nucleases. Similar experiments with the 7SL RNA bound to proteins within the signal recognition partide show essentially the same digestion pattern. A model of the secondary structure common to Drosophila and human 7SL RNA is presented.