Background: Platelet response to activating agents is used to monitor the efficacy of anti-aggregation therapies. The aim of our study has been to demonstrate the existence of relationships between early events of ADP-induced platelet activation, measured by flow cytometry and platelet-rich plasma aggregation,quantified by optical aggregometry. Methods: We evaluated peripheral blood of 12 donors. The following parameters were quantified by cytometry after stimulation with adenosine diphosphate (ADP) (0.5, 1, 2, 5, 10, 20 uM): CD62P (P-selectin) and PAC-1 expression, and cytosolic Ca2+ mobilization. Aggregation was measured by optical aggregometry. We also studied 13 patients, undergoing coronary stenting, treated with aspirin (before procedure) or with aspirin plus clopidogrel (after procedure). We evaluated CD62P and PAC-1 expression, aggregation,and vasodilator-stimulated phopshoprotein phosphorylation (platelet reactivity index, PRI). Results: Flow procedures were more sensitive than aggregometry, with a lowest interindividual variability. Linear relationships existed in donors between CD62P expression and Ca2+ mobilization (P0.0001),and between aggregation and Ca2+ mobilization (P0.0001). Linear relationships existed between aggregation and CD62P expression, as percentage (P0.0001), or relative fluorescence intensity (RFI) (P0.0001). Exponential equations related aggregation and PAC-1 expression, as percentage (P0.0001), or RFI (P0.0001). Linear relationships between aggregation and CD62P expression (as percentage) existed in the patients before (P
Relationships between optical aggregometry (type Born) and flow cytometry in evaluating ADP-induced platelet activation
Sbrana S;
2008
Abstract
Background: Platelet response to activating agents is used to monitor the efficacy of anti-aggregation therapies. The aim of our study has been to demonstrate the existence of relationships between early events of ADP-induced platelet activation, measured by flow cytometry and platelet-rich plasma aggregation,quantified by optical aggregometry. Methods: We evaluated peripheral blood of 12 donors. The following parameters were quantified by cytometry after stimulation with adenosine diphosphate (ADP) (0.5, 1, 2, 5, 10, 20 uM): CD62P (P-selectin) and PAC-1 expression, and cytosolic Ca2+ mobilization. Aggregation was measured by optical aggregometry. We also studied 13 patients, undergoing coronary stenting, treated with aspirin (before procedure) or with aspirin plus clopidogrel (after procedure). We evaluated CD62P and PAC-1 expression, aggregation,and vasodilator-stimulated phopshoprotein phosphorylation (platelet reactivity index, PRI). Results: Flow procedures were more sensitive than aggregometry, with a lowest interindividual variability. Linear relationships existed in donors between CD62P expression and Ca2+ mobilization (P0.0001),and between aggregation and Ca2+ mobilization (P0.0001). Linear relationships existed between aggregation and CD62P expression, as percentage (P0.0001), or relative fluorescence intensity (RFI) (P0.0001). Exponential equations related aggregation and PAC-1 expression, as percentage (P0.0001), or RFI (P0.0001). Linear relationships between aggregation and CD62P expression (as percentage) existed in the patients before (PFile | Dimensione | Formato | |
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Descrizione: Relationships between optical aggregometry (type Born) and flow cytometry in evaluating ADP-induced platelet activation
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