Plant regeneration of Fraxinus angustifolia by in vitro shoot organogenesis

The in vitro regeneration of Fraxinus angustifolia through bud/shoot organogenesis onembryonic explants was investigated. Embryo axes and cotyledons from mature seeds underwentadventitious regeneration after exposure to growth regulators, the embryo axes showing a greaterregenerative potential than cotyledons. While the MS medium proved effective for the inductionand initiation phases, it was inadequate for bud development, and was replaced by DKW medium.A constant supply of benzyladenine (BA) appeared essential for all phases. The gelling agents usedhad an important role in morphogenesis: Duchefa gelrite positively affected the number of newbuds per explant. The rooting of developed shoots was easily achieved on auxin-free WPM mediumand most of the rooted plantlets ``hardened'' after a period of acclimation in a mist greenhouse