We assayed the effects of phenol red (pr), estrogen (Es), and progesterone (Pg) in three-dimensional organotypic cultures of human uterine endocervix. Small intact fragments deposited on sponges submerged in DMEM with 10% fetal bovine serum were cultured in three different conditions: with pr (DMEM(pr+)), without pr (DMEM(pr-)), and without pr but with the addition of physiological concentrations of Es and Pg [DMEM(pr-)(Es+Pg)]. Cell viability and cellular responses were assayed after 4, 10, and 21 days using immunohistochemistry for the expression and distribution of the following markers: mucins and mucopolysaccharides (PAS staining), pan-cytokeratins (AE1/AE3), CK19, p63, Ki-67, vimentin, estrogen receptor-alpha (ER-alpha), and progesterone receptor (PR). The fragments cultivated in DMEM(pr+) showed a cuboidal, poorly differentiated epithelial phenotype and signs of stroma degeneration. In DMEM(pr-), both tissue architecture and cellular heterogeneity were much better preserved: epithelial cells showed a more columnar shape, and stroma was very well conserved, maintaining cell density. The addition of Es and Pg further improved the histology and physiology of the fragments: in DMEM(pr-)(Es+Pg), epithelial cells retained a columnar shape, secreted mucins, and formed areas of squamous hyperplasia.
Cytoarchitecture modifications of the human uterine endocervical mucosa in long-term three-dimensional organotypic culture
2005
Abstract
We assayed the effects of phenol red (pr), estrogen (Es), and progesterone (Pg) in three-dimensional organotypic cultures of human uterine endocervix. Small intact fragments deposited on sponges submerged in DMEM with 10% fetal bovine serum were cultured in three different conditions: with pr (DMEM(pr+)), without pr (DMEM(pr-)), and without pr but with the addition of physiological concentrations of Es and Pg [DMEM(pr-)(Es+Pg)]. Cell viability and cellular responses were assayed after 4, 10, and 21 days using immunohistochemistry for the expression and distribution of the following markers: mucins and mucopolysaccharides (PAS staining), pan-cytokeratins (AE1/AE3), CK19, p63, Ki-67, vimentin, estrogen receptor-alpha (ER-alpha), and progesterone receptor (PR). The fragments cultivated in DMEM(pr+) showed a cuboidal, poorly differentiated epithelial phenotype and signs of stroma degeneration. In DMEM(pr-), both tissue architecture and cellular heterogeneity were much better preserved: epithelial cells showed a more columnar shape, and stroma was very well conserved, maintaining cell density. The addition of Es and Pg further improved the histology and physiology of the fragments: in DMEM(pr-)(Es+Pg), epithelial cells retained a columnar shape, secreted mucins, and formed areas of squamous hyperplasia.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
