The study explores the possibility of olive (Olea europaea L.) germplasm cryopreservation, using both the vitrification and the encapsulation-dehydration procedures, followed by one-step freezing in liquid nitrogen. When shoot tips of olive (cv Frantoio) were incubated at 0°C in the PVS2 vitrification solution for 60 min, a 15% survival was recorded after direct immersion in liquid nitrogen and thawing at 40°C. More than 90% of encapsulated shoot tips and nodal segments survived after a 2-day preculture in 0.5 M-sucrose OM medium and a 4-hour exposure to silica gel, but the treatment was not effective in the protection of explants during ultra-freezing. Best results were obtained with olive embryogenic tissue (cv Canino), as 38% of samples survived to cryopreservation and showed enhanced morphogenicity.
Vitrification of shoot tips, nodal segments and embryogenic tissue of olive (Olea europaea L.) for germplasm cryopreservation
Benelli C;De Carlo A;Lambardi M;
2001
Abstract
The study explores the possibility of olive (Olea europaea L.) germplasm cryopreservation, using both the vitrification and the encapsulation-dehydration procedures, followed by one-step freezing in liquid nitrogen. When shoot tips of olive (cv Frantoio) were incubated at 0°C in the PVS2 vitrification solution for 60 min, a 15% survival was recorded after direct immersion in liquid nitrogen and thawing at 40°C. More than 90% of encapsulated shoot tips and nodal segments survived after a 2-day preculture in 0.5 M-sucrose OM medium and a 4-hour exposure to silica gel, but the treatment was not effective in the protection of explants during ultra-freezing. Best results were obtained with olive embryogenic tissue (cv Canino), as 38% of samples survived to cryopreservation and showed enhanced morphogenicity.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.