The application of an enzymatic assay using the 4-methylumbelliferyl-f}-D-glucuronide (MUG) substrate for the spectrofluorometric détermination of fecal pollution in marine environments is evaluated. Results of the analysis carried out on a total of 80 samples collected from nearshore areas show the présence of significative corrélations between plate (m-FC médium) and microscopical (direct immunofluorescence, IF) counts and the enzymatic methods (r=0.807 and r=0.747 respectively for MUG-FC and MUG-IF). The advantages and the quantitative and qualitative limits to the use of this biochemical assay are discussed.
Rapid detection of Escherichia coli in coastal waters by use of the fluorogenic substrate 4-methylumbelliferyl-b-D-glucuronide: preliminary results
Caruso G;Zaccone R;Crisafi E;Monticelli L
1998
Abstract
The application of an enzymatic assay using the 4-methylumbelliferyl-f}-D-glucuronide (MUG) substrate for the spectrofluorometric détermination of fecal pollution in marine environments is evaluated. Results of the analysis carried out on a total of 80 samples collected from nearshore areas show the présence of significative corrélations between plate (m-FC médium) and microscopical (direct immunofluorescence, IF) counts and the enzymatic methods (r=0.807 and r=0.747 respectively for MUG-FC and MUG-IF). The advantages and the quantitative and qualitative limits to the use of this biochemical assay are discussed.File in questo prodotto:
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