The N-methyl-D-aspartate subtpe of glutamate receptor (NR) is a Iigand-gated ionic channel that pIays an important roIe in synaptic pIasticity, memory and spatial Ierning and is also implicated in the sensitization of pain padways.- For theese properties. it represents a prominent drug targel. Research on NR Iigands and modulators has progressed enormous]y by the use of heterelogous cell systems, in which NR complexes of known, composition are selectively expressed. All functionaI NRs are tetrameric complexes and contain two copies of the essential subunit NR I and two copies of any of the four different NR2s (A. B, C and D); so the heterologous expression of NR channels is complicated by the requirement for two types of subunit.
A Rapid and Efficient Protocol for Transfecting NMDA Receptor Channels into Mammalian Cells
Gavazzo P;Marchetti C
2008
Abstract
The N-methyl-D-aspartate subtpe of glutamate receptor (NR) is a Iigand-gated ionic channel that pIays an important roIe in synaptic pIasticity, memory and spatial Ierning and is also implicated in the sensitization of pain padways.- For theese properties. it represents a prominent drug targel. Research on NR Iigands and modulators has progressed enormous]y by the use of heterelogous cell systems, in which NR complexes of known, composition are selectively expressed. All functionaI NRs are tetrameric complexes and contain two copies of the essential subunit NR I and two copies of any of the four different NR2s (A. B, C and D); so the heterologous expression of NR channels is complicated by the requirement for two types of subunit.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
