Lime witches' broom phytoplasma (16SrII, LWB) causes major losses to lime cultivation in Iran. The role of its immunodominant membrane protein (IMP) in the specific interaction with vector proteins was addressed. The carboxy terminal moiety of LWB IMP, coding the extracellular part of the protein, was expressed (LWB f?IMP) as a tool to study IMP interactions with insect pro- teins. In preliminary affinity chromatography experiments, leafhopper vector proteins with different sizes interacted with LWB f?IMP .
In vitro interactions between immunodominant membrane protein of Lime witches broom phytoplasma and leafhopper vector proteins
Galetto L;
2011
Abstract
Lime witches' broom phytoplasma (16SrII, LWB) causes major losses to lime cultivation in Iran. The role of its immunodominant membrane protein (IMP) in the specific interaction with vector proteins was addressed. The carboxy terminal moiety of LWB IMP, coding the extracellular part of the protein, was expressed (LWB f?IMP) as a tool to study IMP interactions with insect pro- teins. In preliminary affinity chromatography experiments, leafhopper vector proteins with different sizes interacted with LWB f?IMP .File in questo prodotto:
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