Streptococcus thermophilus is a Gram-positive bacterium belonging to the group of lactic acid bacteria, among which several genera play an essential role in manufacture of food products. Recently, a genomic consortium sequenced and annotated its entire genome, which has been demonstrated to contain 1900 coding sequences. In this study, we have revealed the expression products of almost 200 different genes using a proteomic strategy combining 2-DE plus MALDITOF PMF and differential 1-DE plus mLC-ESI-IT-MS/MS. Thus, a number of cellular pathways related to important physiological processes were described at the proteomic level.
PROTEOMIC ANALYSIS OF STREPTOCOCCUS THERMOPHILUS, A PROBIOTIC LACTIC ACID BACTERIUM.
Arena S;Rullo R;Renzone G;D'Ambrosio C;Ledda L;Maglione G;Ferrara L;Scaloni A
2004
Abstract
Streptococcus thermophilus is a Gram-positive bacterium belonging to the group of lactic acid bacteria, among which several genera play an essential role in manufacture of food products. Recently, a genomic consortium sequenced and annotated its entire genome, which has been demonstrated to contain 1900 coding sequences. In this study, we have revealed the expression products of almost 200 different genes using a proteomic strategy combining 2-DE plus MALDITOF PMF and differential 1-DE plus mLC-ESI-IT-MS/MS. Thus, a number of cellular pathways related to important physiological processes were described at the proteomic level.| File | Dimensione | Formato | |
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