The archaeal replication apparatus appears to be a simplified version of the eukaryotic one with fewer polypeptides and simpler protein complexes. Herein, we report evidence that a Cdc6-like factor from the hyper-thermophilic crenarchaeon Sulfolobus solfataricus stimulates binding of the homo-hexameric MCM-like complex to bubble- and fork-containing DNA oligonucleotides that mimic early replication intermediates. This function does not require the Cdc6 ATP- and DNA-binding activities. These findings may provide important clues to understand how the DNA replication initiation process has evolved in the more complex eukaryotic organisms.
A Cdc6-like factor from the archaeon sulfolobus solfataricus promotes binding of MCM to DNA
De Felice M;De Falco M;Rossi M;Pisani FM
2004
Abstract
The archaeal replication apparatus appears to be a simplified version of the eukaryotic one with fewer polypeptides and simpler protein complexes. Herein, we report evidence that a Cdc6-like factor from the hyper-thermophilic crenarchaeon Sulfolobus solfataricus stimulates binding of the homo-hexameric MCM-like complex to bubble- and fork-containing DNA oligonucleotides that mimic early replication intermediates. This function does not require the Cdc6 ATP- and DNA-binding activities. These findings may provide important clues to understand how the DNA replication initiation process has evolved in the more complex eukaryotic organisms.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
