We present our results in the identification of human polymorphisms within the 2 nd exon of the HLA-A region, in the exon 4 of apolipoprotein E and in the exon 15 of the transferrin gene. Allele specific detection was performed by PCR followed by ligase detection reaction (LDR) in combination with a universal array, a powerful method for high throughput DNA sequence analysis. This work confirmed the results previously obtained by sequence or enzymatic analysis thus demonstrating the feasibility of genotyping studies by LDR using a universal DNA array.
Investigation of a subset of human polymorphisms by ligase detection reaction and universal array
Consolandi C;Bordoni R;Castiglioni B;Mezzelani A;De Bellis G
2002
Abstract
We present our results in the identification of human polymorphisms within the 2 nd exon of the HLA-A region, in the exon 4 of apolipoprotein E and in the exon 15 of the transferrin gene. Allele specific detection was performed by PCR followed by ligase detection reaction (LDR) in combination with a universal array, a powerful method for high throughput DNA sequence analysis. This work confirmed the results previously obtained by sequence or enzymatic analysis thus demonstrating the feasibility of genotyping studies by LDR using a universal DNA array.File in questo prodotto:
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