1. CYP2Js have been studied in various mammals but not in sheep, as an animal model used to test veterinary drug metabolism. 2. Sheep CYP2J was cloned from liver mRNA by RACE. The cDNA, after modification at N- and C-terminals, was expressed in E.coli and the sheep CYP2J protein, purified by chromatography, was 80% homologous to human, and monkey CYP2J2. 3. RT-PCR experiments showed that CYP2J mRNA was expressed in liver, cortex, respiratory and olfactory mucosa, heart, bronchi, lung, spleen, small intestine and kidney. 4. The purified enzyme was catalytically active towards aminopyrine, all-trans-retinoic acid and particularly arachidonic acid forming 20-HETE, 19-HETE and 18-HETE (about 86 % of total) and 14,15- , 11,12-, 8,9- and 5-6-EETs (about 14 % of total), with a regioselectivity similar to that shown by the mammalian CYP2J2s.
Molecular cloning and enzymatic characterization of sheep CYP2J
Longo V
2010
Abstract
1. CYP2Js have been studied in various mammals but not in sheep, as an animal model used to test veterinary drug metabolism. 2. Sheep CYP2J was cloned from liver mRNA by RACE. The cDNA, after modification at N- and C-terminals, was expressed in E.coli and the sheep CYP2J protein, purified by chromatography, was 80% homologous to human, and monkey CYP2J2. 3. RT-PCR experiments showed that CYP2J mRNA was expressed in liver, cortex, respiratory and olfactory mucosa, heart, bronchi, lung, spleen, small intestine and kidney. 4. The purified enzyme was catalytically active towards aminopyrine, all-trans-retinoic acid and particularly arachidonic acid forming 20-HETE, 19-HETE and 18-HETE (about 86 % of total) and 14,15- , 11,12-, 8,9- and 5-6-EETs (about 14 % of total), with a regioselectivity similar to that shown by the mammalian CYP2J2s.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
