A halo-alkaliphilic, Gram-positive, non-motile bacterium, designated strain 4AG(T), was isolated from a mineral pool located in Malvizza, Campania, southern Italy. On the basis of 16S rRNA gene sequence analysis, strain 4AG(T) was shown to belong to the genus Alkalibacillus within the phylum Firmicutes; its phylogenetic distance from recognized Alkalibacillus species was < 95(.)0 %. Chemotaxonomic data (MK-7 as the major menaquinone; directly cross-linked meso-diaminopimelic acid in the cell wall; phosphatidylglycerol and diphosphatidylglycerol as major polar lipids; iso-C-15:0, anteiso-C-15:0 and iso-C-17:0 as major fatty acids; and glycine betaine and glutamate as major compatible solutes) supported the affiliation of the strain to the genus Alkalibacillus. The results of DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 4AG(T) from the two recognized Alkalibacillus species. Strain 4AG(T) therefore represents a novel species, for which the name Alkalibacillus filiformis sp. nov. is proposed. The type strain is 4AG(T) (=DSM 15448(T)=ATCC BAA-956(T)).
Bacillus saliphilus sp nov., isolated from a mineral pool in Campania, Italy
Romano I;Lama L;Nicolaus B;Gambacorta A;Giordano A
2005
Abstract
A halo-alkaliphilic, Gram-positive, non-motile bacterium, designated strain 4AG(T), was isolated from a mineral pool located in Malvizza, Campania, southern Italy. On the basis of 16S rRNA gene sequence analysis, strain 4AG(T) was shown to belong to the genus Alkalibacillus within the phylum Firmicutes; its phylogenetic distance from recognized Alkalibacillus species was < 95(.)0 %. Chemotaxonomic data (MK-7 as the major menaquinone; directly cross-linked meso-diaminopimelic acid in the cell wall; phosphatidylglycerol and diphosphatidylglycerol as major polar lipids; iso-C-15:0, anteiso-C-15:0 and iso-C-17:0 as major fatty acids; and glycine betaine and glutamate as major compatible solutes) supported the affiliation of the strain to the genus Alkalibacillus. The results of DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 4AG(T) from the two recognized Alkalibacillus species. Strain 4AG(T) therefore represents a novel species, for which the name Alkalibacillus filiformis sp. nov. is proposed. The type strain is 4AG(T) (=DSM 15448(T)=ATCC BAA-956(T)).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
