Sequence-tagged sites (STS), specified by primer pairs and their corresponding PCR products, have become a fundamental component of Human Genome initiative. Biological separation of chromosomes is possible with rodent-human somatic cell hybrids containing all or sub-regions of a single human chromosome. STSs can then be derived from human specific Alu-Alu fragments or form human specific plasmids, lambda clones, cosmids or YACs. An alternative is provided by physical separation of chromosome of interest by flow-sorting. We have analyzed both X-specific YAC end-sequences and fragments of sorted chromosomes as sources of STSs.
SEQUENCE-TAGGED SITES (STSS) FROM YAC INSERT-ENDS AND X-SPECIFIC FLOW-SORTED CHROMOSOMES
CICCODICOLA, AMethodology
;CINTI, C;ESPOSITO, T;CAMPANILE, C;
1994
Abstract
Sequence-tagged sites (STS), specified by primer pairs and their corresponding PCR products, have become a fundamental component of Human Genome initiative. Biological separation of chromosomes is possible with rodent-human somatic cell hybrids containing all or sub-regions of a single human chromosome. STSs can then be derived from human specific Alu-Alu fragments or form human specific plasmids, lambda clones, cosmids or YACs. An alternative is provided by physical separation of chromosome of interest by flow-sorting. We have analyzed both X-specific YAC end-sequences and fragments of sorted chromosomes as sources of STSs.File in questo prodotto:
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