Embryogenic calli formed from fertilized ovules of carob dissected from small legumes (20-25 mm in length) when cultured on MS (Murashige and Skoog) medium supplemented with sucrose (150mM), malt extract (500 mg l(-1)) and 6-benzylaminopurine (13.3 mu M). Nonaberrant somatic embryos formed when those calli were cultured onto MS medium supplemented with 85mM sucrose and 0.45 mu M 2,4-dichlorophenoxyacetic acid. Upon transfer into half-strength MS medium supplemented with sucrose (60mM) and indole-3-butyric acid (10 mu M), these embryos germinated into plantlets. AII attempts to induce embryogenic callus from unfertilized ovules were unsuccessful.
Callus induction and somatic embryogenesis in carob (Ceratonia siliqua L.) from ovule culture
Carimi F;
1997
Abstract
Embryogenic calli formed from fertilized ovules of carob dissected from small legumes (20-25 mm in length) when cultured on MS (Murashige and Skoog) medium supplemented with sucrose (150mM), malt extract (500 mg l(-1)) and 6-benzylaminopurine (13.3 mu M). Nonaberrant somatic embryos formed when those calli were cultured onto MS medium supplemented with 85mM sucrose and 0.45 mu M 2,4-dichlorophenoxyacetic acid. Upon transfer into half-strength MS medium supplemented with sucrose (60mM) and indole-3-butyric acid (10 mu M), these embryos germinated into plantlets. AII attempts to induce embryogenic callus from unfertilized ovules were unsuccessful.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
