An experimental study was carried out, aimed at optimizing the opto-geometric configuration for measuring the concentration of biologicalcells by means of static light scattering measurements. A LED-based optoelectronic setup making use of optical fibers was experimented,as the precursor of a low-cost device to be integrated in instrumentation for cytometry. Two biological sample types were considered astest samples of the most popular analyses - cervical cells and urine, respectively. The most suitable wavelengths and detecting angles wereidentified, and calibration curves were calculated.
Light scattering measurements for quantifying biological cell concentration: an optimization of opto-geometric parameters
A. G. Mignani;L. Ciaccheri
;A. A. Mencaglia
2012
Abstract
An experimental study was carried out, aimed at optimizing the opto-geometric configuration for measuring the concentration of biologicalcells by means of static light scattering measurements. A LED-based optoelectronic setup making use of optical fibers was experimented,as the precursor of a low-cost device to be integrated in instrumentation for cytometry. Two biological sample types were considered astest samples of the most popular analyses - cervical cells and urine, respectively. The most suitable wavelengths and detecting angles wereidentified, and calibration curves were calculated.File in questo prodotto:
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Descrizione: Light scattering measurements for quantifying biological cell concentration: an optimization of opto-geometric parameters
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