We investigated the expression of the partially duplicated alpha 7 nAChR subunit gene in three monocytic cell lines: THP-1, U937 and Mono-Mac-6. Qualitative PCR revealed the presence of the classic alpha 7 gene in these lineages, but real-time PCR showed the exclusive expression of alpha 7dup. Both mRNA and protein levels were reduced in THP-1 upon LPS challenge, and it was found that transcriptional down-regulation was mediated by a direct mechanism dependent on NF-kappa B as its specific inhibitor parthenolide prevented the reduction in the alpha 7dup transcript Such precise regulation suggests that alpha 7dup may specifically participate in the inflammatory response of the innate immune system.
Expression of the alpha7 nAChR subunit duplicate form (CHRFAM7A) is down-regulated in the monocytic cell line THP-1 on treatment with LPS.
Benfante R;Gotti C;Clementi F;Fornasari;
2011
Abstract
We investigated the expression of the partially duplicated alpha 7 nAChR subunit gene in three monocytic cell lines: THP-1, U937 and Mono-Mac-6. Qualitative PCR revealed the presence of the classic alpha 7 gene in these lineages, but real-time PCR showed the exclusive expression of alpha 7dup. Both mRNA and protein levels were reduced in THP-1 upon LPS challenge, and it was found that transcriptional down-regulation was mediated by a direct mechanism dependent on NF-kappa B as its specific inhibitor parthenolide prevented the reduction in the alpha 7dup transcript Such precise regulation suggests that alpha 7dup may specifically participate in the inflammatory response of the innate immune system.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
