Two bacterial strains, VI.14 and VIII.04T, were isolated from the Mediterranean sponge Axinella verrucosa collected off the Israeli coast near Sdot Yam. The non-motile aerobic Gram-negative isolates were oxidase-negative and catalase-positive, and formed golden-brown colonies on marine agar 2216. The pigment was neither diffusible and nor Flexirubin-like. Strain VIII.04T grew at 15-37 °C, at pH 6.0-9.0, in presence of 20-50 g L-1 NaCl, and 20-80 g L-1 sea salts, whereas the spectrum was narrower for strain VI.14 with growth at pH 7.0-8.0 and in the presence of 30-50 g L-1 NaCl and 30-70 g L-1 sea salts. The predominant fatty acid (>50%) in both strains was iso-C15:0, and the major respiratory quinone was MK-6. The DNA G+C content was 30.7 and 31.1 mol% for VIII.04T and VI.14, respectively. Results from 16S rDNA sequence analysis indicated that both strains are closest related to members of the family Flavobacteriaceae within the phylum Bacteroidetes, with as much as 91.7% 16S rDNA sequence similarity. On the basis of the polyphasic data, we suggest that the strains represent a novel genus and species within the Flavobacteriaceae, for which the name Aureivirga marina gen. nov., sp. nov. is proposed. Strain VIII.04T (= ATCC BAA-2394 = LMG 26721) is the type strain.
Aureivirga marina gen. nov., sp. nov., a novel marine bacterium isolated from the Mediterranean sponge Axinella verrucosa
Giordano A;Orlando P;Gambacorta A;
2013
Abstract
Two bacterial strains, VI.14 and VIII.04T, were isolated from the Mediterranean sponge Axinella verrucosa collected off the Israeli coast near Sdot Yam. The non-motile aerobic Gram-negative isolates were oxidase-negative and catalase-positive, and formed golden-brown colonies on marine agar 2216. The pigment was neither diffusible and nor Flexirubin-like. Strain VIII.04T grew at 15-37 °C, at pH 6.0-9.0, in presence of 20-50 g L-1 NaCl, and 20-80 g L-1 sea salts, whereas the spectrum was narrower for strain VI.14 with growth at pH 7.0-8.0 and in the presence of 30-50 g L-1 NaCl and 30-70 g L-1 sea salts. The predominant fatty acid (>50%) in both strains was iso-C15:0, and the major respiratory quinone was MK-6. The DNA G+C content was 30.7 and 31.1 mol% for VIII.04T and VI.14, respectively. Results from 16S rDNA sequence analysis indicated that both strains are closest related to members of the family Flavobacteriaceae within the phylum Bacteroidetes, with as much as 91.7% 16S rDNA sequence similarity. On the basis of the polyphasic data, we suggest that the strains represent a novel genus and species within the Flavobacteriaceae, for which the name Aureivirga marina gen. nov., sp. nov. is proposed. Strain VIII.04T (= ATCC BAA-2394 = LMG 26721) is the type strain.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
