Beauvericin (BEA), a supercritical fluid extraction with supercritical carbon dioxide from maize was investigated. Extraction efficiencies under several different extraction conditions were examined. Pressure, temperature, extraction time, organic modifier and water matrix content (10%) were investigated. The best extraction conditions were at a temperature of 60 degreesC, 3200 psi, for 30 min static extraction time and methanol as modifier solvent. Extraction recovery of 36% without modifier by adding water to the matrix in the extraction vessel (reproducibility relative standard deviations (R.S.D.) = 3-5%) were recorded. Extraction recovery of 76.9% with methanol as co-solvent (reproducibility R.S.D. = 3-5%) was obtained. Data shows that SFE gives a lower BEA recovery compared to conventional extraction protocol with organic solvents while SFE with modifier and conventional extraction yields are comparable. BEA extract contents were determined by high pressure liquid chromatography (HPLC) with a diode array detector (DAD) at 205 nm and BEA peak confirmed by LC-MS. Acetonitrile-water as mobile phase and column G 18 were both tested. Instrumental and analytical parameters were optimized in the range linear interval from 1 to 500 mg kg(-1) and reached a detection limit of 2 ng. (C) 2003 Elsevier Ltd. All rights reserved.
Supercritical fluid extraction of Beauvericin from maize
Logrieco A;
2004
Abstract
Beauvericin (BEA), a supercritical fluid extraction with supercritical carbon dioxide from maize was investigated. Extraction efficiencies under several different extraction conditions were examined. Pressure, temperature, extraction time, organic modifier and water matrix content (10%) were investigated. The best extraction conditions were at a temperature of 60 degreesC, 3200 psi, for 30 min static extraction time and methanol as modifier solvent. Extraction recovery of 36% without modifier by adding water to the matrix in the extraction vessel (reproducibility relative standard deviations (R.S.D.) = 3-5%) were recorded. Extraction recovery of 76.9% with methanol as co-solvent (reproducibility R.S.D. = 3-5%) was obtained. Data shows that SFE gives a lower BEA recovery compared to conventional extraction protocol with organic solvents while SFE with modifier and conventional extraction yields are comparable. BEA extract contents were determined by high pressure liquid chromatography (HPLC) with a diode array detector (DAD) at 205 nm and BEA peak confirmed by LC-MS. Acetonitrile-water as mobile phase and column G 18 were both tested. Instrumental and analytical parameters were optimized in the range linear interval from 1 to 500 mg kg(-1) and reached a detection limit of 2 ng. (C) 2003 Elsevier Ltd. All rights reserved.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
