Aqueous ozone treatments were applied to tomato plants (cv. Roma VF) before and after Meloidogyne incognita infection in controlled growth conditions. Ozonated water was produced in situ by an ozone generator at 8 ppm. Treatments were applied for 3 and 4 days as soil drench at the rate of 10 ml/pot (50 ml). Untreated plants were used as control. The sensitivity to aqueous ozone treatments was verified by the production of reactive oxygen species (ROS) in roots. A significant ROS production was observed after two days of ozone treatments in comparison to the untreated plants, without adversely influencing the growth of the treated plants. Visual examination of the root systems revealed no symptomatic evidence of root browning or other damages indicative of oxidative stress. Tomato plants infected with M. incognita before and after ozone treatments were grown in a glasshouse at 25 ± 2°C. After 60 days the plants were uprooted, and dry shoot and root fresh weights were recorded. Root gall index, eggs and juveniles/g root, total nematode population density and reproduction rates were evaluated. Root gall index was assessed according to a 0-10 scale, whereas nematodes were extracted from roots and soil by centrifugation and the Coolen's method, respectively. Reproduction rate was calculated by the ratio between final and initial nematode population density (r =Pf/Pi). Data from the experiments were subjected to ANOVA and means compared by Least Significant Difference's test. Root gall index, eggs and juveniles/g root, total nematode population density and the reproduction rate were significantly reduced in both ozone treatments (3 and 4 days of treatments), either before or after nematode inoculation, in comparison to the untreated control.
Response Of Meloidogyne Incognita Infected Tomato Plants To Aqueous Ozone Treatments
Melillo MT;Veronico P;Laquale S;Sasanelli N
2014
Abstract
Aqueous ozone treatments were applied to tomato plants (cv. Roma VF) before and after Meloidogyne incognita infection in controlled growth conditions. Ozonated water was produced in situ by an ozone generator at 8 ppm. Treatments were applied for 3 and 4 days as soil drench at the rate of 10 ml/pot (50 ml). Untreated plants were used as control. The sensitivity to aqueous ozone treatments was verified by the production of reactive oxygen species (ROS) in roots. A significant ROS production was observed after two days of ozone treatments in comparison to the untreated plants, without adversely influencing the growth of the treated plants. Visual examination of the root systems revealed no symptomatic evidence of root browning or other damages indicative of oxidative stress. Tomato plants infected with M. incognita before and after ozone treatments were grown in a glasshouse at 25 ± 2°C. After 60 days the plants were uprooted, and dry shoot and root fresh weights were recorded. Root gall index, eggs and juveniles/g root, total nematode population density and reproduction rates were evaluated. Root gall index was assessed according to a 0-10 scale, whereas nematodes were extracted from roots and soil by centrifugation and the Coolen's method, respectively. Reproduction rate was calculated by the ratio between final and initial nematode population density (r =Pf/Pi). Data from the experiments were subjected to ANOVA and means compared by Least Significant Difference's test. Root gall index, eggs and juveniles/g root, total nematode population density and the reproduction rate were significantly reduced in both ozone treatments (3 and 4 days of treatments), either before or after nematode inoculation, in comparison to the untreated control.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
