A 90K Custom microarray was employed to highlight the effects of Pentachlorophenol on Drosophila melanogaster transcriptome in order to identify potential metabolic pathways involved in detoxification of polyhalogenated aromatic hydrocarbons. Microarray assays were performed on mRNA from early third instar larvae challenged with different PCP concentrations (0, 20 and 2000 ppm) incorporated in dietary supply. Bayesian Estimation of Temporal Regulation (BETR) analysis identified a set of 514 Differentially Expressed (DE) mRNAs among the control and the treated larvae (p<= 0.05). The DE genes encoding enzymes potentially involved in detoxification included numerous cytochrome P450 genes (Cyp). In particular 7 Cyp genes were up-regulated (Cyp6a2, Cyp 6a8,Cyp6t1, Cyp4d14, Cyp9A14, Cyp6a9, Cyp28d2) and 2 were down-regulated (Cyp4d2, Cyp317a1). Overexpression was also detected in several genes of the glutathione-mediated detoxification pathway: two glutathione-S-transferases (GSTE10 and GSTS1), a gamma-glutamyltranspeptidase and a puromycin-sensitive aminopeptidase (alanyl aminopeptidase).
Investigating candidate genes for Pentachlorophenol detoxification in Drosophila melanogaster
Monti MM;Pedata PA;
2013
Abstract
A 90K Custom microarray was employed to highlight the effects of Pentachlorophenol on Drosophila melanogaster transcriptome in order to identify potential metabolic pathways involved in detoxification of polyhalogenated aromatic hydrocarbons. Microarray assays were performed on mRNA from early third instar larvae challenged with different PCP concentrations (0, 20 and 2000 ppm) incorporated in dietary supply. Bayesian Estimation of Temporal Regulation (BETR) analysis identified a set of 514 Differentially Expressed (DE) mRNAs among the control and the treated larvae (p<= 0.05). The DE genes encoding enzymes potentially involved in detoxification included numerous cytochrome P450 genes (Cyp). In particular 7 Cyp genes were up-regulated (Cyp6a2, Cyp 6a8,Cyp6t1, Cyp4d14, Cyp9A14, Cyp6a9, Cyp28d2) and 2 were down-regulated (Cyp4d2, Cyp317a1). Overexpression was also detected in several genes of the glutathione-mediated detoxification pathway: two glutathione-S-transferases (GSTE10 and GSTS1), a gamma-glutamyltranspeptidase and a puromycin-sensitive aminopeptidase (alanyl aminopeptidase).I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
