Contaminating substances accumulated in the environment as a result of solid and liquid landfills, industrial and agricultural activities. Many of these substances are composed of chlorinated aromatics, bio-accumulative, stable molecules, which affect various levels of the food chain. This is the case of the Pentachlorophenol (PCP) that has been used extensively, but due to its toxicity and bioaccumulation, it has become a contaminant. Several strategies are studied to remediate polluted environments, based on physical and chemical methods or involving biological agents (Bioremediation). Bioremediation, as a spontaneous or controlled strategy, is usually considered a low-impact and sustainable methodology, compared to traditional clean-up techniques, and, consequently, ideal for recovering polluted ecosystems. Well known bioremediation agents include plants and microorganisms, as well as their metabolites and enzymes. In contrast, animals such as insects, despite their remarkable versatility in developing resistance to xenobiotics, are much less exploited as a source of enzymes potentially useful in bioremediation programs. In order to fill this gap, we chose to study the effects of Pentachlorophenol (PCP) on Drosophila melanogaster.Potential metabolic pathways in Drosophila melanogaster involved in detoxification of polyhalogenated aromatic hydrocarbons were investigated A 90K Custom microarray was employed to highlight the effects of Pentachlorophenol on transcriptome in order to identify. Microarray assays were performed on mRNA from early third instar larvae challenged with different PCP concentrations (0, 20 and 2000 ppm) incorporated in dietary supply. Linear Model for Microarray Data (LIMMA) analysis identified a set of 663 Differentially Expressed (DE) mRNAs among the control and the treated larvae (p<= 0.05). The DE genes encoding enzymes potentially involved in detoxification included numerous cytochrome P450 genes (Cyp). In particular 8 Cyp genes were up-regulated (Cyp6a2, Cyp6a8, Cyp6a17, Cyp6d2, Cyp4d14, Cyp28d2, Cyp12a5, Cyp12e1) and 1 was down-regulated (Cyp4d2). Overexpression was also detected in genes of the glutathione-mediated detoxification pathway: three glutathione-S-transferases (GSTD4, GSTD9, GSTS1), and a puromycin-sensitive aminopeptidase (alanyl aminopeptidase)
Drosophila melanogaster pentachlorophenol induced genes
Monti MM;Pedata PA;
2013
Abstract
Contaminating substances accumulated in the environment as a result of solid and liquid landfills, industrial and agricultural activities. Many of these substances are composed of chlorinated aromatics, bio-accumulative, stable molecules, which affect various levels of the food chain. This is the case of the Pentachlorophenol (PCP) that has been used extensively, but due to its toxicity and bioaccumulation, it has become a contaminant. Several strategies are studied to remediate polluted environments, based on physical and chemical methods or involving biological agents (Bioremediation). Bioremediation, as a spontaneous or controlled strategy, is usually considered a low-impact and sustainable methodology, compared to traditional clean-up techniques, and, consequently, ideal for recovering polluted ecosystems. Well known bioremediation agents include plants and microorganisms, as well as their metabolites and enzymes. In contrast, animals such as insects, despite their remarkable versatility in developing resistance to xenobiotics, are much less exploited as a source of enzymes potentially useful in bioremediation programs. In order to fill this gap, we chose to study the effects of Pentachlorophenol (PCP) on Drosophila melanogaster.Potential metabolic pathways in Drosophila melanogaster involved in detoxification of polyhalogenated aromatic hydrocarbons were investigated A 90K Custom microarray was employed to highlight the effects of Pentachlorophenol on transcriptome in order to identify. Microarray assays were performed on mRNA from early third instar larvae challenged with different PCP concentrations (0, 20 and 2000 ppm) incorporated in dietary supply. Linear Model for Microarray Data (LIMMA) analysis identified a set of 663 Differentially Expressed (DE) mRNAs among the control and the treated larvae (p<= 0.05). The DE genes encoding enzymes potentially involved in detoxification included numerous cytochrome P450 genes (Cyp). In particular 8 Cyp genes were up-regulated (Cyp6a2, Cyp6a8, Cyp6a17, Cyp6d2, Cyp4d14, Cyp28d2, Cyp12a5, Cyp12e1) and 1 was down-regulated (Cyp4d2). Overexpression was also detected in genes of the glutathione-mediated detoxification pathway: three glutathione-S-transferases (GSTD4, GSTD9, GSTS1), and a puromycin-sensitive aminopeptidase (alanyl aminopeptidase)I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
