Molecular markers are very efficient tools for evaluating inter-and intraspecific genetic differences among fungi. Results obtained using Random Amplified Polymorphic DNA markers (RAPDs) for the characterisation of about 100 species of edible mushrooms commercially important in Italy, i.e. Pleurotus eryngii, P. ostreatus, P. nebrodensis, P. cornucopiae, P. opuntiae, Agaricus bisporus and A. bitorquis are reported. The analysis of polymorphism has been carried out on total DNA isolated from mycelia grown in submerged culture. Specific fragments of mitochondrial rDNA small- and large subunits were successfully amplified by PCR. The analysis of mit-rDNA seems to be a promising technique for the molecular systematics of fungi, since different levels of polymorphism of amplified regions of small and large subunits of mitochondrial rDNA can be revealed. The S1-MS2 region is rather conservative and could serve as a molecular marker for genus-level phylogenetic studies, while the ML regions allow discrimination at interspecific level. Results concerning the MS1-MS2 region reveal the specific patterns for the genera Pleurotus, Agrocybe and Lentinula, while those concerning the ML1-ML4 region permit the separation of the Pleurotus species into 3 groups: 1) P. eryngii and P. ostreatus; 2) P. cornucopiae, SMR0120, P. pulmonarius, P. sajor-caju and P. sapidus; 3) P. cornucopiae SMR 0237, P. ulmarius and P. citrimopilestus.
Molecular Fingerprinting of Pleurotus and Agaricus Germplasm
Laddomada B;
1998
Abstract
Molecular markers are very efficient tools for evaluating inter-and intraspecific genetic differences among fungi. Results obtained using Random Amplified Polymorphic DNA markers (RAPDs) for the characterisation of about 100 species of edible mushrooms commercially important in Italy, i.e. Pleurotus eryngii, P. ostreatus, P. nebrodensis, P. cornucopiae, P. opuntiae, Agaricus bisporus and A. bitorquis are reported. The analysis of polymorphism has been carried out on total DNA isolated from mycelia grown in submerged culture. Specific fragments of mitochondrial rDNA small- and large subunits were successfully amplified by PCR. The analysis of mit-rDNA seems to be a promising technique for the molecular systematics of fungi, since different levels of polymorphism of amplified regions of small and large subunits of mitochondrial rDNA can be revealed. The S1-MS2 region is rather conservative and could serve as a molecular marker for genus-level phylogenetic studies, while the ML regions allow discrimination at interspecific level. Results concerning the MS1-MS2 region reveal the specific patterns for the genera Pleurotus, Agrocybe and Lentinula, while those concerning the ML1-ML4 region permit the separation of the Pleurotus species into 3 groups: 1) P. eryngii and P. ostreatus; 2) P. cornucopiae, SMR0120, P. pulmonarius, P. sajor-caju and P. sapidus; 3) P. cornucopiae SMR 0237, P. ulmarius and P. citrimopilestus.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
