tIn this paper we propose polysaccharide hydrogels combining alginate (ALG) and hyaluronan (HA) asbiofunctional platform for dermal wound repair. Hydrogels produced by internal gelation were homo-geneous and easy to handle. Rheological evaluation of gelation kinetics of ALG/HA mixtures at differentratios allowed understanding the HA effect on ALG cross-linking process. Disk-shaped hydrogels, at differ-ent ALG/HA ratio, were characterized for morphology, homogeneity and mechanical properties. Resultssuggest that, although the presence of HA does significantly slow down gelation kinetics, the concen-tration of cross-links reached at the end of gelation is scarcely affected. The in vitro activity of ALG/HAdressings was tested on adipose derived multipotent adult stem cells (Ad-MSC) and an immortalizedkeratinocyte cell line (HaCaT). Hydrogels did not interfere with cell viability in both cells lines, but sig-nificantly promoted gap closure in a scratch assay at early (1 day) and late (5 days) stages as comparedto hydrogels made of ALG alone (p < 0.01 and 0.001 for Ad-MSC and HaCaT, respectively). In vivo woundhealing studies, conducted on a rat model of excised wound indicated that after 5 days ALG/HA hydrogelssignificantly promoted wound closure as compared to ALG ones (p < 0.001). Overall results demonstratethat the integration of HA in a physically cross-linked ALG hydrogel can be a versatile strategy to promotewound healing that can be easily translated in a clinical setting.
Alginate-hyaluronan hybrid hydrogels for wound healing
V D'Esposito;MR Ambrosio;P Russo;P Formisano;
2015
Abstract
tIn this paper we propose polysaccharide hydrogels combining alginate (ALG) and hyaluronan (HA) asbiofunctional platform for dermal wound repair. Hydrogels produced by internal gelation were homo-geneous and easy to handle. Rheological evaluation of gelation kinetics of ALG/HA mixtures at differentratios allowed understanding the HA effect on ALG cross-linking process. Disk-shaped hydrogels, at differ-ent ALG/HA ratio, were characterized for morphology, homogeneity and mechanical properties. Resultssuggest that, although the presence of HA does significantly slow down gelation kinetics, the concen-tration of cross-links reached at the end of gelation is scarcely affected. The in vitro activity of ALG/HAdressings was tested on adipose derived multipotent adult stem cells (Ad-MSC) and an immortalizedkeratinocyte cell line (HaCaT). Hydrogels did not interfere with cell viability in both cells lines, but sig-nificantly promoted gap closure in a scratch assay at early (1 day) and late (5 days) stages as comparedto hydrogels made of ALG alone (p < 0.01 and 0.001 for Ad-MSC and HaCaT, respectively). In vivo woundhealing studies, conducted on a rat model of excised wound indicated that after 5 days ALG/HA hydrogelssignificantly promoted wound closure as compared to ALG ones (p < 0.001). Overall results demonstratethat the integration of HA in a physically cross-linked ALG hydrogel can be a versatile strategy to promotewound healing that can be easily translated in a clinical setting.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.